8TFK

Cryo-EM structure of the Methanosarcina mazei glutamine synthetase (GS) with Met-Sox-P and ADP

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	Glutamine synthetase	polymer	467	52827.9	12	UniProt (Q8PY99) Pfam (PF03951) Pfam (PF00120) In PDB	Methanosarcina mazei Go1	GS,Glutamate--ammonia ligase
2	D, I, J, E, A...	MAGNESIUM ION	non-polymer		24.3	36	Chemie (MG)
3	I, D, J, E, K...	L-METHIONINE-S-SULFOXIMINE PHOSPHATE	non-polymer		260.2	12	Chemie (P3S)
4	C, I, D, J, E...	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	12	Chemie (ADP)

Sequence modifications

A, B, C, D, E, F, G, H, I, J, K, L: 1 - 447 (UniProt: Q8PY99)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	633935.1
Non-Polymers*	Number of molecules	60
	Total formula weight	9123.9
All*	Total formula weight	643059.0

*Water molecules are not included.