Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

8TFK

Cryo-EM structure of the Methanosarcina mazei glutamine synthetase (GS) with Met-Sox-P and ADP

This is a non-PDB format compatible entry.

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0004356	molecular_function	glutamine synthetase activity
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0006542	biological_process	glutamine biosynthetic process
A	0006598	biological_process	polyamine catabolic process
A	0006807	biological_process	obsolete nitrogen compound metabolic process
A	0016874	molecular_function	ligase activity
A	0046872	molecular_function	metal ion binding
B	0003824	molecular_function	catalytic activity
B	0004356	molecular_function	glutamine synthetase activity
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0006542	biological_process	glutamine biosynthetic process
B	0006598	biological_process	polyamine catabolic process
B	0006807	biological_process	obsolete nitrogen compound metabolic process
B	0016874	molecular_function	ligase activity
B	0046872	molecular_function	metal ion binding
C	0003824	molecular_function	catalytic activity
C	0004356	molecular_function	glutamine synthetase activity
C	0005524	molecular_function	ATP binding
C	0005737	cellular_component	cytoplasm
C	0006542	biological_process	glutamine biosynthetic process
C	0006598	biological_process	polyamine catabolic process
C	0006807	biological_process	obsolete nitrogen compound metabolic process
C	0016874	molecular_function	ligase activity
C	0046872	molecular_function	metal ion binding
D	0003824	molecular_function	catalytic activity
D	0004356	molecular_function	glutamine synthetase activity
D	0005524	molecular_function	ATP binding
D	0005737	cellular_component	cytoplasm
D	0006542	biological_process	glutamine biosynthetic process
D	0006598	biological_process	polyamine catabolic process
D	0006807	biological_process	obsolete nitrogen compound metabolic process
D	0016874	molecular_function	ligase activity
D	0046872	molecular_function	metal ion binding
E	0003824	molecular_function	catalytic activity
E	0004356	molecular_function	glutamine synthetase activity
E	0005524	molecular_function	ATP binding
E	0005737	cellular_component	cytoplasm
E	0006542	biological_process	glutamine biosynthetic process
E	0006598	biological_process	polyamine catabolic process
E	0006807	biological_process	obsolete nitrogen compound metabolic process
E	0016874	molecular_function	ligase activity
E	0046872	molecular_function	metal ion binding
F	0003824	molecular_function	catalytic activity
F	0004356	molecular_function	glutamine synthetase activity
F	0005524	molecular_function	ATP binding
F	0005737	cellular_component	cytoplasm
F	0006542	biological_process	glutamine biosynthetic process
F	0006598	biological_process	polyamine catabolic process
F	0006807	biological_process	obsolete nitrogen compound metabolic process
F	0016874	molecular_function	ligase activity
F	0046872	molecular_function	metal ion binding
G	0003824	molecular_function	catalytic activity
G	0004356	molecular_function	glutamine synthetase activity
G	0005524	molecular_function	ATP binding
G	0005737	cellular_component	cytoplasm
G	0006542	biological_process	glutamine biosynthetic process
G	0006598	biological_process	polyamine catabolic process
G	0006807	biological_process	obsolete nitrogen compound metabolic process
G	0016874	molecular_function	ligase activity
G	0046872	molecular_function	metal ion binding
H	0003824	molecular_function	catalytic activity
H	0004356	molecular_function	glutamine synthetase activity
H	0005524	molecular_function	ATP binding
H	0005737	cellular_component	cytoplasm
H	0006542	biological_process	glutamine biosynthetic process
H	0006598	biological_process	polyamine catabolic process
H	0006807	biological_process	obsolete nitrogen compound metabolic process
H	0016874	molecular_function	ligase activity
H	0046872	molecular_function	metal ion binding
I	0003824	molecular_function	catalytic activity
I	0004356	molecular_function	glutamine synthetase activity
I	0005524	molecular_function	ATP binding
I	0005737	cellular_component	cytoplasm
I	0006542	biological_process	glutamine biosynthetic process
I	0006598	biological_process	polyamine catabolic process
I	0006807	biological_process	obsolete nitrogen compound metabolic process
I	0016874	molecular_function	ligase activity
I	0046872	molecular_function	metal ion binding
J	0003824	molecular_function	catalytic activity
J	0004356	molecular_function	glutamine synthetase activity
J	0005524	molecular_function	ATP binding
J	0005737	cellular_component	cytoplasm
J	0006542	biological_process	glutamine biosynthetic process
J	0006598	biological_process	polyamine catabolic process
J	0006807	biological_process	obsolete nitrogen compound metabolic process
J	0016874	molecular_function	ligase activity
J	0046872	molecular_function	metal ion binding
K	0003824	molecular_function	catalytic activity
K	0004356	molecular_function	glutamine synthetase activity
K	0005524	molecular_function	ATP binding
K	0005737	cellular_component	cytoplasm
K	0006542	biological_process	glutamine biosynthetic process
K	0006598	biological_process	polyamine catabolic process
K	0006807	biological_process	obsolete nitrogen compound metabolic process
K	0016874	molecular_function	ligase activity
K	0046872	molecular_function	metal ion binding
L	0003824	molecular_function	catalytic activity
L	0004356	molecular_function	glutamine synthetase activity
L	0005524	molecular_function	ATP binding
L	0005737	cellular_component	cytoplasm
L	0006542	biological_process	glutamine biosynthetic process
L	0006598	biological_process	polyamine catabolic process
L	0006807	biological_process	obsolete nitrogen compound metabolic process
L	0016874	molecular_function	ligase activity
L	0046872	molecular_function	metal ion binding

Functional Information from PROSITE/UniProt

site_id	PS00180
Number of Residues	19
Details	GLNA_1 Glutamine synthetase signature 1. FDGSSiqgftrieESDmkL

Chain	Residue	Details
A	PHE56-LEU74

site_id	PS00181
Number of Residues	16
Details	GLNA_ATP Glutamine synthetase putative ATP-binding region signature. KPLfgv..NGSGmHsnqS

Chain	Residue	Details
A	LYS237-SER252

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	84
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:P12425

Chain	Residue	Details
A	GLU135
B	GLU192
B	GLU199
B	GLY244
B	HIS248
B	GLU336
C	GLU135
C	GLU137
C	GLU192
C	GLU199
C	GLY244
A	GLU137
C	HIS248
C	GLU336
D	GLU135
D	GLU137
D	GLU192
D	GLU199
D	GLY244
D	HIS248
D	GLU336
E	GLU135
A	GLU192
E	GLU137
E	GLU192
E	GLU199
E	GLY244
E	HIS248
E	GLU336
F	GLU135
F	GLU137
F	GLU192
F	GLU199
A	GLU199
F	GLY244
F	HIS248
F	GLU336
G	GLU135
G	GLU137
G	GLU192
G	GLU199
G	GLY244
G	HIS248
G	GLU336
A	GLY244
H	GLU135
H	GLU137
H	GLU192
H	GLU199
H	GLY244
H	HIS248
H	GLU336
I	GLU135
I	GLU137
I	GLU192
A	HIS248
I	GLU199
I	GLY244
I	HIS248
I	GLU336
J	GLU135
J	GLU137
J	GLU192
J	GLU199
J	GLY244
J	HIS248
A	GLU336
J	GLU336
K	GLU135
K	GLU137
K	GLU192
K	GLU199
K	GLY244
K	HIS248
K	GLU336
L	GLU135
L	GLU137
B	GLU135
L	GLU192
L	GLU199
L	GLY244
L	HIS248
L	GLU336
B	GLU137

site_id	SWS_FT_FI2
Number of Residues	48
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:P9WN39

Chain	Residue	Details
A	GLU187
C	ASN243
C	ARG319
C	ARG324
D	GLU187
D	ASN243
D	ARG319
D	ARG324
E	GLU187
E	ASN243
E	ARG319
A	ASN243
E	ARG324
F	GLU187
F	ASN243
F	ARG319
F	ARG324
G	GLU187
G	ASN243
G	ARG319
G	ARG324
H	GLU187
A	ARG319
H	ASN243
H	ARG319
H	ARG324
I	GLU187
I	ASN243
I	ARG319
I	ARG324
J	GLU187
J	ASN243
J	ARG319
A	ARG324
J	ARG324
K	GLU187
K	ASN243
K	ARG319
K	ARG324
L	GLU187
L	ASN243
L	ARG319
L	ARG324
B	GLU187
B	ASN243
B	ARG319
B	ARG324
C	GLU187

site_id	SWS_FT_FI3
Number of Residues	12
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:P77961

Chain	Residue	Details
A	SER252
J	SER252
K	SER252
L	SER252
B	SER252
C	SER252
D	SER252
E	SER252
F	SER252
G	SER252
H	SER252
I	SER252

site_id	SWS_FT_FI4
Number of Residues	36
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:P0A1P6

Chain	Residue	Details
A	ARG301
D	ARG301
D	GLU307
D	ARG338
E	ARG301
E	GLU307
E	ARG338
F	ARG301
F	GLU307
F	ARG338
G	ARG301
A	GLU307
G	GLU307
G	ARG338
H	ARG301
H	GLU307
H	ARG338
I	ARG301
I	GLU307
I	ARG338
J	ARG301
J	GLU307
A	ARG338
J	ARG338
K	ARG301
K	GLU307
K	ARG338
L	ARG301
L	GLU307
L	ARG338
B	ARG301
B	GLU307
B	ARG338
C	ARG301
C	GLU307
C	ARG338

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		Validation report (PDF)