8T5C

Lassa GPC Trimer in complex with Fab 8.11G and nanobody D5

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E (B, A, C)	Glycoprotein G1	polymer	202	22884.9	3	UniProt (P08669) Pfam (PF00798)	Lassa virus Josiah	GP1
2	B, D, F (b, a, c)	Glycoprotein G2	polymer	194	22143.2	3	UniProt (P08669) Pfam (PF00798)	Lassa virus Josiah	GP2
3	G (D)	D5 nanobody	polymer	120	13228.5	1		Camelus bactrianus
4	H, J (H, E)	8.11G Heavy Chain	polymer	120	13257.6	2		Homo sapiens
5	I, K (L, I)	8.11G Light Chain	polymer	109	12101.5	2		Homo sapiens
6	L (F)	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1397.2	1	In PDB GlyTouCan (G80966KZ)
7	AA, M, T, X (X, G, Q, U)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	4	In PDB GlyTouCan (G15407YE)
8	N, O, Y (J, K, V)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		383.3	3	In PDB GlyTouCan (G90333CG)
9	P, U, Z (M, R, W)	alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1073.0	3	In PDB GlyTouCan (G09724ZC)
10	Q, V (N, S)	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	2	In PDB GlyTouCan (G22768VO)
11	R (O)	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	1	In PDB GlyTouCan (G42227JK)
12	S (P)	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose	branched		504.4	1	In PDB GlyTouCan (G00619DD)
13	W (T)	alpha-D-mannopyranose-(1-6)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1073.0	1	In PDB GlyTouCan (G34442SS)
14	BA, CA, DA, EA, FA... (B, b, A, a, C...)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	23	Chemie (NAG)

Sequence modifications

B, A, C: 59 - 257 (UniProt: P08669)

PDB	External Database	Details
Gly 206	Arg 207	conflict
Cys 207	-	insertion
Arg 258	-	expression tag
Arg 259	-	expression tag

b, a, c: 260 - 418 (UniProt: P08669)

PDB	External Database	Details
Cys 326	Leu 326	conflict
Pro 329	Glu 329	conflict
Gly 419	-	expression tag
Gly 420	-	expression tag
Gly 421	-	expression tag
Tyr 422	-	expression tag
Ile 423	-	expression tag
Pro 424	-	expression tag
Glu 425	-	expression tag
Ala 426	-	expression tag
Pro 427	-	expression tag
Arg 428	-	expression tag
Asp 429	-	expression tag
Gly 430	-	expression tag
Gln 431	-	expression tag
Ala 432	-	expression tag
Tyr 433	-	expression tag
Val 434	-	expression tag
Arg 435	-	expression tag
Lys 436	-	expression tag
Asp 437	-	expression tag
Gly 438	-	expression tag
Glu 439	-	expression tag
Trp 440	-	expression tag
Val 441	-	expression tag
Leu 442	-	expression tag
Leu 443	-	expression tag
Ser 444	-	expression tag
Thr 445	-	expression tag
Phe 446	-	expression tag
Leu 447	-	expression tag
Gly 448	-	expression tag
Gly 449	-	expression tag
Leu 450	-	expression tag
Val 451	-	expression tag
Pro 452	-	expression tag
Arg 453	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	11
	Total formula weight	199030.9
Branched	Number of molecules	16
	Total formula weight	12422.2
Non-Polymers*	Number of molecules	23
	Total formula weight	5087.8
All*	Total formula weight	216540.9

*Water molecules are not included.