8T3X

TNA polymerase, closed ternary

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	10-92, TNA polymerase	polymer	760	88459.5	1	UniProt (D0VWU9)	Thermococcus kodakarensis
2	B (T)	Template	polymer	15	4586.0	1		synthetic construct
3	C (P)	Primer	polymer	12	3633.4	1		synthetic construct
4	D (A)	[(3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-oxolan-3-yl] [oxidanyl(phosphonooxy)phosphoryl] hydrogen phosphate	non-polymer		477.2	1	Chemie (9O7)
5	E (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
6	F (A)	4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID	non-polymer		238.3	1	Chemie (EPE)
7	G, H, I (A, T, P)	water	water		18.0	47	Chemie (HOH)

Sequence modifications

A: 1 - 760 (UniProt: D0VWU9)

PDB	External Database	Details
Arg 99	Lys 99	engineered mutation
Ala 102	Glu 102	engineered mutation
Val 107	Ile 107	engineered mutation
Ile 127	Val 127	engineered mutation
Thr 136	Lys 136	engineered mutation
Ala 141	Asp 141	engineered mutation
Ala 143	Glu 143	engineered mutation
His 147	Glu 147	engineered mutation
Lys 285	Gln 285	engineered mutation
Ala 296	Thr 296	engineered mutation
Gln 297	Thr 297	engineered mutation
Gly 304	Asn 304	engineered mutation
Val 337	Ile 337	engineered mutation
His 339	Gln 339	engineered mutation
Pro 340	Ser 340	engineered mutation
Tyr 356	Phe 356	engineered mutation
Arg 375	Lys 375	engineered mutation
Tyr 377	Leu 377	engineered mutation
Glu 378	Ala 378	engineered mutation
Leu 381	-	insertion
Glu 383	Gln 382	engineered mutation
Ala 386	Glu 385	engineered mutation
Lys 395	Arg 394	engineered mutation
Arg 466	Lys 465	engineered mutation
Val 472	Ile 471	engineered mutation
Leu 474	Pro 473	engineered mutation
Leu 475	Ile 474	engineered mutation
Lys 477	Arg 476	engineered mutation
Arg 486	Ala 485	engineered mutation
Ser 492	Asn 491	engineered mutation
Gly 493	Ser 492	engineered mutation
Gln 520	Glu 519	engineered mutation
Glu 523	Thr 522	engineered mutation
Thr 524	Met 523	engineered mutation
Arg 527	Lys 526	engineered mutation
Phe 533	Tyr 532	engineered mutation
Leu 538	Ile 537	engineered mutation
Ala 540	Ser 539	engineered mutation
Pro 548	Thr 547	engineered mutation
His 550	Pro 549	engineered mutation
Lys 562	Met 561	engineered mutation
Asp 566	Lys 565	engineered mutation
Leu 575	Ala 574	engineered mutation
Lys 585	Glu 584	engineered mutation
Asp 602	Gly 601	engineered mutation
Gly 607	Arg 606	engineered mutation
Gly 615	Asp 614	engineered mutation
Arg 672	Lys 671	engineered mutation
Ser 717	Pro 716	engineered mutation
Ala 724	Thr 723	engineered mutation
Pro 741	Ala 740	engineered mutation
Cys 749	Phe 748	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	96678.8
Non-Polymers*	Number of molecules	3
	Total formula weight	739.8
All*	Total formula weight	97418.6

*Water molecules are not included.