8T3X
TNA polymerase, closed ternary
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-10-27 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 76.869, 99.525, 110.727 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.390 - 2.730 |
R-factor | 0.247 |
Rwork | 0.242 |
R-free | 0.30140 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.452 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.390 | 2.828 |
High resolution limit [Å] | 2.730 | 2.730 |
Rmerge | 0.177 | 0.970 |
Number of reflections | 23008 | 2237 |
<I/σ(I)> | 11.36 | |
Completeness [%] | 99.0 | |
Redundancy | 6.6 | |
CC(1/2) | 0.766 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.2 M Lithium chloride , 0.1 M HEPES pH 6.5, 17.6% PEG 6000 |