8T3O

Cryo-EM structure of the TUG-891 bound FFA4-Gq complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	Free fatty acid receptor 4	polymer	297	33517.3	1	UniProt (Q5NUL3) Pfam (PF00001)	Homo sapiens (human)	G-protein coupled receptor 120,G-protein coupled receptor 129,G-protein coupled receptor GT01,G-protein coupled receptor PGR4,Omega-3 fatty acid receptor 1
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	342	37516.9	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	57	6261.2	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (A)	Guanine nucleotide-binding protein G(q)	polymer	230	26670.3	1		Homo sapiens
5	E (N)	scFv16	polymer	266	28634.8	1		Mus musculus
6	F (R)	3-{4-[(4-fluoro-4'-methyl[1,1'-biphenyl]-2-yl)methoxy]phenyl}propanoic acid	non-polymer		364.4	1	Chemie (YN9)
7	G (R)	(2R)-1-{[(R)-hydroxy{[(1R,2R,3R,4R,5S,6R)-2,3,5,6-tetrahydroxy-4-(phosphonooxy)cyclohexyl]oxy}phosphoryl]oxy}-3-(octadecanoyloxy)propan-2-yl (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoate	non-polymer		967.1	1	Chemie (2Y5)
8	H (R)	PALMITIC ACID	non-polymer		256.4	1	Chemie (PLM)

Sequence modifications

R: 22 - 328 (UniProt: Q5NUL3)

PDB	External Database	Details
-	Val 147	deletion
-	Arg 148	deletion
-	Gly 149	deletion
-	Leu 184	deletion
-	Pro 185	deletion
-	Gly 186	deletion
-	Ala 187	deletion
-	Asp 188	deletion
-	Gln 189	deletion
-	Glu 190	deletion

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Gly 341	-	expression tag
Ser 342	-	expression tag
Ser 343	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	132600.5
Non-Polymers*	Number of molecules	3
	Total formula weight	1587.9
All*	Total formula weight	134188.4

*Water molecules are not included.