8T25

Cryo-EM structure of the RBD-ACE2 interface of the SARS-CoV-2 trimeric spike protein bound to ACE2 receptor after local refinement at downRBD conformation.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Angiotensin-converting enzyme	polymer	771	89375.5	1	UniProt (A0A7T0Q2W2) Pfam (PF01401) Pfam (PF16959)	Neovison vison
2	B (B)	Spike glycoprotein	polymer	208	23370.2	1	UniProt (P0DTC2) Pfam (PF09408)	Severe acute respiratory syndrome coronavirus 2

Sequence modifications

A: 1 - 739 (UniProt: A0A7T0Q2W2)

PDB	External Database	Details
Gly 740	-	expression tag
Ser 741	-	expression tag
Gly 742	-	expression tag
Ser 743	-	expression tag
Gly 744	-	expression tag
Ser 745	-	expression tag
Gly 746	-	expression tag
His 747	-	expression tag
His 748	-	expression tag
His 749	-	expression tag
His 750	-	expression tag
His 751	-	expression tag
His 752	-	expression tag
Gly 753	-	expression tag
Ser 754	-	expression tag
Gly 755	-	expression tag
Ser 756	-	expression tag
Gly 757	-	expression tag
Leu 758	-	expression tag
Asn 759	-	expression tag
Asp 760	-	expression tag
Ile 761	-	expression tag
Phe 762	-	expression tag
Glu 763	-	expression tag
Ala 764	-	expression tag
Gln 765	-	expression tag
Lys 766	-	expression tag
Ile 767	-	expression tag
Glu 768	-	expression tag
Trp 769	-	expression tag
His 770	-	expression tag
Glu 771	-	expression tag

B: 321 - 528 (UniProt: P0DTC2)

PDB	External Database	Details
Phe 453	Tyr 453	variant

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	112745.7
All*	Total formula weight	112745.7

*Water molecules are not included.