8SZH
Cryo-EM structure of cinacalcet-bound human calcium-sensing receptor CaSR-Gi complex in lipid nanodiscs
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Extracellular calcium-sensing receptor | polymer | 886 | 99929.1 | 2 | UniProt (P41180) Pfam (PF01094) Pfam (PF07562) Pfam (PF00003) | Homo sapiens (human) | CaR,CaSR,hCasR,Parathyroid cell calcium-sensing receptor 1,PCaR1 |
| 2 | C (C) | Guanine nucleotide-binding protein G(i) subunit alpha-3 | polymer | 354 | 40585.1 | 1 | UniProt (P08754) Pfam (PF00503) | Homo sapiens (human) | G(i) alpha-3 |
| 3 | D (D) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 343 | 37574.0 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (human) | |
| 4 | E (E) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 5 | F, G, H (F, G, H) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 3 | In PDB GlyTouCan (G42666HT) | |||
| 6 | I, S (A, B) | N-[(1R)-1-(naphthalen-1-yl)ethyl]-3-[3-(trifluoromethyl)phenyl]propan-1-amine | non-polymer | 357.4 | 2 | Chemie (YP4) | |||
| 7 | J, T (A, B) | 1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE | non-polymer | 787.1 | 2 | Chemie (PCW) | |||
| 8 | K, U, V (A, B) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 3 | Chemie (NAG) | |||
| 9 | L, M, W, X (A, B) | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) | |||
| 10 | N, Y (A, B) | TRYPTOPHAN | non-polymer | 204.2 | 2 | Chemie (TRP) | |||
| 11 | O, Z (A, B) | PHOSPHATE ION | non-polymer | 95.0 | 2 | Chemie (PO4) | |||
| 12 | AA, BA, P, Q (B, A) | SPERMINE | non-polymer | 202.3 | 4 | Chemie (SPM) | |||
| 13 | R (B) | CHOLESTEROL | non-polymer | 386.7 | 1 | Chemie (CLR) | |||
| 14 | CA (B) | water | water | 18.0 | 1 | Chemie (HOH) |
Sequence modifications
A, B: 19 - 894 (UniProt: P41180)
D: 2 - 340 (UniProt: P62873)
| PDB | External Database | Details |
|---|---|---|
| Asp 9 | - | expression tag |
| Tyr 10 | - | expression tag |
| Lys 11 | - | expression tag |
| Asp 12 | - | expression tag |
| Asp 13 | - | expression tag |
| Asp 14 | - | expression tag |
| Asp 15 | - | expression tag |
| Lys 16 | - | expression tag |
| Ala 17 | - | expression tag |
| Ala 18 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Gly 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 285878.5 | |
| Branched | Number of molecules | 3 |
| Total formula weight | 1273.2 | |
| Non-Polymers* | Number of molecules | 20 |
| Total formula weight | 4907.4 | |
| All* | Total formula weight | 292059.1 |
