8SZH
Cryo-EM structure of cinacalcet-bound human calcium-sensing receptor CaSR-Gi complex in lipid nanodiscs
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004930 | molecular_function | G protein-coupled receptor activity |
| A | 0007186 | biological_process | G protein-coupled receptor signaling pathway |
| A | 0016020 | cellular_component | membrane |
| B | 0004930 | molecular_function | G protein-coupled receptor activity |
| B | 0007186 | biological_process | G protein-coupled receptor signaling pathway |
| B | 0016020 | cellular_component | membrane |
| C | 0000139 | cellular_component | Golgi membrane |
| C | 0001664 | molecular_function | G protein-coupled receptor binding |
| C | 0003924 | molecular_function | GTPase activity |
| C | 0005515 | molecular_function | protein binding |
| C | 0005525 | molecular_function | GTP binding |
| C | 0005654 | cellular_component | nucleoplasm |
| C | 0005730 | cellular_component | nucleolus |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005765 | cellular_component | lysosomal membrane |
| C | 0005789 | cellular_component | endoplasmic reticulum membrane |
| C | 0005794 | cellular_component | Golgi apparatus |
| C | 0005813 | cellular_component | centrosome |
| C | 0005834 | cellular_component | heterotrimeric G-protein complex |
| C | 0005856 | cellular_component | cytoskeleton |
| C | 0005886 | cellular_component | plasma membrane |
| C | 0007165 | biological_process | signal transduction |
| C | 0007186 | biological_process | G protein-coupled receptor signaling pathway |
| C | 0007188 | biological_process | adenylate cyclase-modulating G protein-coupled receptor signaling pathway |
| C | 0007193 | biological_process | adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway |
| C | 0007194 | biological_process | negative regulation of adenylate cyclase activity |
| C | 0007212 | biological_process | G protein-coupled dopamine receptor signaling pathway |
| C | 0016020 | cellular_component | membrane |
| C | 0016239 | biological_process | positive regulation of macroautophagy |
| C | 0019001 | molecular_function | guanyl nucleotide binding |
| C | 0019003 | molecular_function | GDP binding |
| C | 0030496 | cellular_component | midbody |
| C | 0031683 | molecular_function | G-protein beta/gamma-subunit complex binding |
| C | 0046039 | biological_process | GTP metabolic process |
| C | 0046872 | molecular_function | metal ion binding |
| C | 0051301 | biological_process | cell division |
| C | 0070062 | cellular_component | extracellular exosome |
| D | 0001750 | cellular_component | photoreceptor outer segment |
| D | 0003924 | molecular_function | GTPase activity |
| D | 0005515 | molecular_function | protein binding |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0005765 | cellular_component | lysosomal membrane |
| D | 0005829 | cellular_component | cytosol |
| D | 0005834 | cellular_component | heterotrimeric G-protein complex |
| D | 0005886 | cellular_component | plasma membrane |
| D | 0007165 | biological_process | signal transduction |
| D | 0007186 | biological_process | G protein-coupled receptor signaling pathway |
| D | 0007191 | biological_process | adenylate cyclase-activating dopamine receptor signaling pathway |
| D | 0007200 | biological_process | phospholipase C-activating G protein-coupled receptor signaling pathway |
| D | 0007213 | biological_process | G protein-coupled acetylcholine receptor signaling pathway |
| D | 0007265 | biological_process | Ras protein signal transduction |
| D | 0008283 | biological_process | cell population proliferation |
| D | 0016020 | cellular_component | membrane |
| D | 0030159 | molecular_function | signaling receptor complex adaptor activity |
| D | 0044877 | molecular_function | protein-containing complex binding |
| D | 0045202 | cellular_component | synapse |
| D | 0050909 | biological_process | sensory perception of taste |
| D | 0051020 | molecular_function | GTPase binding |
| D | 0060041 | biological_process | retina development in camera-type eye |
| D | 0070062 | cellular_component | extracellular exosome |
| D | 0071380 | biological_process | cellular response to prostaglandin E stimulus |
| D | 0071870 | biological_process | cellular response to catecholamine stimulus |
| D | 0097381 | cellular_component | photoreceptor disc membrane |
| D | 1903561 | cellular_component | extracellular vesicle |
| E | 0005515 | molecular_function | protein binding |
| E | 0005834 | cellular_component | heterotrimeric G-protein complex |
| E | 0005886 | cellular_component | plasma membrane |
| E | 0007165 | biological_process | signal transduction |
| E | 0007186 | biological_process | G protein-coupled receptor signaling pathway |
| E | 0007191 | biological_process | adenylate cyclase-activating dopamine receptor signaling pathway |
| E | 0016020 | cellular_component | membrane |
| E | 0031681 | molecular_function | G-protein beta-subunit binding |
| E | 0048144 | biological_process | fibroblast proliferation |
| E | 0070062 | cellular_component | extracellular exosome |
| E | 0071380 | biological_process | cellular response to prostaglandin E stimulus |
| E | 0071870 | biological_process | cellular response to catecholamine stimulus |
Functional Information from PROSITE/UniProt
| site_id | PS00678 |
| Number of Residues | 15 |
| Details | WD_REPEATS_1 Trp-Asp (WD) repeats signature. LVSAsqDgKLIIWDS |
| Chain | Residue | Details |
| D | LEU70-SER84 | |
| D | ILE157-ILE171 | |
| D | LEU285-ALA299 |
| site_id | PS00979 |
| Number of Residues | 19 |
| Details | G_PROTEIN_RECEP_F3_1 G-protein coupled receptors family 3 signature 1. VaNLLgLFyIPQVSyASSS |
| Chain | Residue | Details |
| A | VAL153-SER171 |
| site_id | PS00980 |
| Number of Residues | 25 |
| Details | G_PROTEIN_RECEP_F3_2 G-protein coupled receptors family 3 signature 2. CCFeCveCpdgeYsdet.DasACnkC |
| Chain | Residue | Details |
| A | CYS561-CYS585 |
| site_id | PS00981 |
| Number of Residues | 11 |
| Details | G_PROTEIN_RECEP_F3_3 G-protein coupled receptors family 3 signature 3. FNEAKfITFSM |
| Chain | Residue | Details |
| A | PHE801-MET811 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | MOD_RES: N-acetylalanine => ECO:0007744|PubMed:19413330, ECO:0007744|PubMed:22814378 |
| Chain | Residue | Details |
| E | ALA2 | |
| C | LEU175 | |
| C | ASN269 | |
| A | TYR829-VAL836 | |
| B | TYR20-PHE612 | |
| B | GLU671-GLN681 | |
| B | ALA746-SER769 | |
| B | TYR829-VAL836 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 1 |
| Details | MOD_RES: Cysteine methyl ester => ECO:0000250|UniProtKB:P63212 |
| Chain | Residue | Details |
| E | CYS68 | |
| C | THR181 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | LIPID: S-geranylgeranyl cysteine => ECO:0000250|UniProtKB:P63212 |
| Chain | Residue | Details |
| E | CYS68 | |
| A | ARG701-GLN724 | |
| A | LYS793-LYS805 | |
| B | LYS636-GLU649 | |
| B | ARG701-GLN724 | |
| B | LYS793-LYS805 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0000305|PubMed:19478087, ECO:0007744|PDB:2V4Z |
| Chain | Residue | Details |
| C | ASP200 | |
| B | LEU650-GLY670 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0007744|PDB:2IHB, ECO:0007744|PDB:2ODE, ECO:0007744|PDB:2V4Z, ECO:0007744|PDB:4G5O, ECO:0007744|PDB:4G5R |
| Chain | Residue | Details |
| C | ALA326 | |
| B | PRO682-ASN700 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 1 |
| Details | MOD_RES: ADP-ribosylarginine; by cholera toxin => ECO:0000250 |
| Chain | Residue | Details |
| C | ARG178 | |
| B | PHE725-THR745 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 1 |
| Details | MOD_RES: Deamidated glutamine; by Photorhabdus PAU_02230 => ECO:0000269|PubMed:24141704 |
| Chain | Residue | Details |
| C | GLN204 | |
| B | LEU770-PHE792 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 1 |
| Details | MOD_RES: ADP-ribosylcysteine; by pertussis toxin => ECO:0000250 |
| Chain | Residue | Details |
| C | CYS351 | |
| B | PHE806-THR828 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 1 |
| Details | LIPID: N-myristoyl glycine => ECO:0000269|PubMed:25255805 |
| Chain | Residue | Details |
| C | GLY2 | |
| B | GLU837-PHE862 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 1 |
| Details | LIPID: S-palmitoyl cysteine => ECO:0000250 |
| Chain | Residue | Details |
| C | CYS3 | |
| B | ASP234 | |
| B | ARG415 | |
| B | GLY557 | |
| A | THR145 | |
| A | GLU231 | |
| A | ASP234 | |
| A | ARG415 | |
| A | GLY557 | |
| B | ARG66 | |
| B | THR145 | |
| B | GLU231 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:27434672, ECO:0000305|PubMed:27386547, ECO:0007744|PDB:5FBH, ECO:0007744|PDB:5FBK, ECO:0007744|PDB:5K5S |
| Chain | Residue | Details |
| A | ILE81 | |
| A | LEU87 | |
| A | LEU88 | |
| B | ILE81 | |
| B | LEU87 | |
| B | LEU88 |
| site_id | SWS_FT_FI12 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:27434672, ECO:0000305|PubMed:27386547, ECO:0007744|PDB:5FBK, ECO:0007744|PDB:5K5S |
| Chain | Residue | Details |
| A | SER84 | |
| B | SER84 |
| site_id | SWS_FT_FI13 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:27434672, ECO:0007744|PDB:5K5S, ECO:0007744|PDB:5K5T |
| Chain | Residue | Details |
| A | THR100 | |
| B | THR100 |
| site_id | SWS_FT_FI14 |
| Number of Residues | 8 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:27386547, ECO:0000269|PubMed:27434672, ECO:0007744|PDB:5FBH, ECO:0007744|PDB:5FBK, ECO:0007744|PDB:5K5S |
| Chain | Residue | Details |
| A | SER147 | |
| A | ALA168 | |
| A | SER170 | |
| A | GLU297 | |
| B | SER147 | |
| B | ALA168 | |
| B | SER170 | |
| B | GLU297 |
| site_id | SWS_FT_FI15 |
| Number of Residues | 2 |
| Details | SITE: Important for ability of agonist AMG 416 to activate G-protein-coupled receptor activity => ECO:0000269|PubMed:26290606 |
| Chain | Residue | Details |
| A | CYS482 | |
| B | CYS482 |
| site_id | SWS_FT_FI16 |
| Number of Residues | 8 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255 |
| Chain | Residue | Details |
| A | ASN90 | |
| A | ASN130 | |
| A | ASN386 | |
| A | ASN400 | |
| B | ASN90 | |
| B | ASN130 | |
| B | ASN386 | |
| B | ASN400 |
| site_id | SWS_FT_FI17 |
| Number of Residues | 14 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255, ECO:0000269|PubMed:27434672 |
| Chain | Residue | Details |
| A | ASN261 | |
| B | ASN446 | |
| B | ASN468 | |
| B | ASN488 | |
| B | ASN541 | |
| B | ASN594 | |
| A | ASN287 | |
| A | ASN446 | |
| A | ASN468 | |
| A | ASN488 | |
| A | ASN541 | |
| A | ASN594 | |
| B | ASN261 | |
| B | ASN287 |
