8SY6

E. coli DNA-directed RNA polymerase transcription elongation complex bound the unnatural dB-UTP base pair in the active site

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	N	Non-template single stranded DNA	polymer	18	5663.7	1		synthetic construct
2	T	Template single stranded DNA	polymer	29	8739.6	1		synthetic construct
3	G, A	DNA-directed RNA polymerase subunit alpha	polymer	329	36558.7	2	UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB	Escherichia coli	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
4	I	DNA-directed RNA polymerase subunit beta	polymer	1342	150820.9	1	UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) In PDB	Escherichia coli	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
5	J	DNA-directed RNA polymerase subunit beta'	polymer	1430	158105.7	1	UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB	Escherichia coli	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
6	R	RNA oligomer	polymer	9	2934.8	1		synthetic construct
7	K	DNA-directed RNA polymerase subunit omega	polymer	91	10249.5	1	UniProt (P0A800) Pfam (PF01192) In PDB	Escherichia coli	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
8	T	2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE	non-polymer		347.2	1	Chemie (DGP)
9	J	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
10	J	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
11	J	URIDINE 5'-TRIPHOSPHATE	non-polymer		484.1	1	Chemie (UTP)

Sequence modifications

J: 1 - 1407 (UniProt: P0A8T7)

PDB	External Database	Details
Leu 1408	-	expression tag
Glu 1409	-	expression tag
Leu 1410	-	expression tag
Glu 1411	-	expression tag
Val 1412	-	expression tag
Leu 1413	-	expression tag
Phe 1414	-	expression tag
Gln 1415	-	expression tag
Gly 1416	-	expression tag
Pro 1417	-	expression tag
Ser 1418	-	expression tag
Ser 1419	-	expression tag
Gly 1420	-	expression tag
His 1421	-	expression tag
His 1422	-	expression tag
His 1423	-	expression tag
His 1424	-	expression tag
His 1425	-	expression tag
His 1426	-	expression tag
His 1427	-	expression tag
His 1428	-	expression tag
His 1429	-	expression tag
His 1430	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	409631.6
Non-Polymers*	Number of molecules	5
	Total formula weight	986.5
All*	Total formula weight	410618.1

*Water molecules are not included.