8SV4
7-Deazapurines and 5-Halogenpyrimidine DNA duplex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Ribonuclease H | polymer | 142 | 16329.5 | 1 | UniProt (Q9KEI9) | Halalkalibacterium halodurans | RNase H |
| 2 | B, C (B, C) | DNA (5'-D(*(B86)P*(7DD)P*(B86)P*(7DD)P*(7DA)P*(7DA)P*(UCL)P*(UCL)P*(B86)P*(7DD)P*(B86)P*(7DD))-3') | polymer | 12 | 3690.3 | 2 | synthetic construct | ||
| 3 | D (A) | 2-[2-(2-ethoxyethoxy)ethoxy]ethanol | non-polymer | 178.2 | 1 | Chemie (FWN) | |||
| 4 | E, F (A) | ACETATE ION | non-polymer | 59.0 | 2 | Chemie (ACT) | |||
| 5 | G, H (A) | SULFATE ION | non-polymer | 96.1 | 2 | Chemie (SO4) | |||
| 6 | I (A) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 1 | Chemie (EDO) | |||
| 7 | J, K, L (A, B, C) | water | water | 18.0 | 79 | Chemie (HOH) |
Sequence modifications
A: 59 - 196 (UniProt: Q9KEI9)
| PDB | External Database | Details |
|---|---|---|
| Gly 55 | - | expression tag |
| Ser 56 | - | expression tag |
| His 57 | - | expression tag |
| Met 58 | - | expression tag |
| Asn 132 | Asp 132 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 23710.0 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 550.5 | |
| All* | Total formula weight | 24260.6 |
