8SUT

Crystal structure of YisK from Bacillus subtilis in complex with reaction product 4-Hydroxy-2-oxoglutaric acid

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Fumarylacetoacetate hydrolase family protein	polymer	312	34550.4	2	UniProt (A0A0D6X359) Pfam (PF01557) UniProt (by SIFTS) (O06724)	Bacillus subtilis
2	C, F (A, B)	(2~{R})-2-oxidanyl-4-oxidanylidene-pentanedioic acid	non-polymer		162.1	2	Chemie (IO9)
3	D, E (A, B)	MANGANESE (II) ION	non-polymer		54.9	2	Chemie (MN)
4	G, H (A, B)	water	water		18.0	148	Chemie (HOH)

Sequence modifications

A, B: 1 - 301 (UniProt: A0A0D6X359)

PDB	External Database	Details
Met -2	-	initiating methionine
Ala -1	-	expression tag
Ser 0	-	expression tag
Leu 302	-	expression tag
Glu 303	-	expression tag
His 304	-	expression tag
His 305	-	expression tag
His 306	-	expression tag
His 307	-	expression tag
His 308	-	expression tag
His 309	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	69100.9
Non-Polymers*	Number of molecules	4
	Total formula weight	434.1
All*	Total formula weight	69535.0

*Water molecules are not included.