8SUT
Crystal structure of YisK from Bacillus subtilis in complex with reaction product 4-Hydroxy-2-oxoglutaric acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2019-10-12 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.686, 93.983, 124.981 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.040 - 1.930 |
| R-factor | 0.18491 |
| Rwork | 0.183 |
| R-free | 0.22990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.360 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (2000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.040 | 1.980 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.068 | 1.500 |
| Rpim | 0.035 | 0.921 |
| Number of reflections | 47426 | 2885 |
| <I/σ(I)> | 12.4 | |
| Completeness [%] | 98.2 | |
| Redundancy | 4.6 | |
| CC(1/2) | 0.998 | 0.259 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.2 M Mg-acetate and 20% (w/v) PEG 3350 |
