8STD

S127A variant of LarB, a carboxylase/hydrolase involved in synthesis of the cofactor for lactate racemase, in complex with authentic substrate NaAD and soaked with CS2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	Pyridinium-3,5-biscarboxylic acid mononucleotide synthase	polymer	256	26494.2	6	UniProt (F9UST0) Pfam (PF00731) In PDB	Lactiplantibacillus plantarum WCFS1	P2CMN synthase,Lactate racemase accessory protein LarB,Lactate racemase activation protein LarB,Lactate racemase maturation protein LarB,Lactate racemization operon protein LarB,Nickel-pincer cofactor biosynthesis protein LarB,Nicotinic acid adenine dinucleotide carboxylase/hydrolase,NaAD carboxylase/hydrolase
2	A, B, C, D, E...	MAGNESIUM ION	non-polymer		24.3	9	Chemie (MG)
3	A, B, C, D, E...	NICOTINIC ACID ADENINE DINUCLEOTIDE	non-polymer		665.4	6	Chemie (DND)

Sequence modifications

A, B, C, D, E, F: 1 - 246 (UniProt: F9UST0)

PDB	External Database	Details
Ala 127	Ser 127	conflict
Ala 247	-	expression tag
Ser 248	-	expression tag
Trp 249	-	expression tag
Ser 250	-	expression tag
His 251	-	expression tag
Pro 252	-	expression tag
Gln 253	-	expression tag
Phe 254	-	expression tag
Glu 255	-	expression tag
Lys 256	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	158965.4
Non-Polymers*	Number of molecules	15
	Total formula weight	4211.3
All*	Total formula weight	163176.6

*Water molecules are not included.