8ST4

The 2alpha3beta stoichiometry of human alpha4beta2 nicotinic acetylcholine receptor in complex with acetylcholine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D	Neuronal acetylcholine receptor subunit alpha-4	polymer	386	44862.4	2	UniProt (P43681) Pfam (PF02931) Pfam (PF02932) In PDB	Homo sapiens (human)
2	B, C, E	Neuronal acetylcholine receptor subunit beta-2	polymer	403	46748.9	3	UniProt (P17787) Pfam (PF02931) Pfam (PF02932) In PDB	Homo sapiens (human)
3	F, H, J	IgG1 Kappa Light Chain	polymer	238	26378.6	3		Mus musculus (mouse)
4	G, I, K	IgG1 Heavy Chain	polymer	462	51195.7	3		Mus musculus (mouse)
5	L, M, N	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	3	In PDB GlyTouCan (G15407YE)
6	A, D	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	6	Chemie (NAG)
7	A, D	ACETYLCHOLINE	non-polymer		146.2	2	Chemie (ACH)
8	B	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
9		water	water		18.0	7	Chemie (HOH)

Sequence modifications

A, D: 1 - 338 (UniProt: P43681)
A, D: 339 - 386 (UniProt: P43681)

PDB	External Database	Details
Glu 343	-	insertion
Arg 344	-	insertion

B, C, E: 1 - 330 (UniProt: P17787)
B, C, E: 331 - 393 (UniProt: P17787)

PDB	External Database	Details
Glu 335	-	insertion
Arg 336	-	insertion
Ser 394	-	linker
Ala 395	-	linker
Trp 396	-	expression tag
Ser 397	-	expression tag
His 398	-	expression tag
Pro 399	-	expression tag
Gln 400	-	expression tag
Phe 401	-	expression tag
Glu 402	-	expression tag
Lys 403	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	11
	Total formula weight	462694.1
Branched	Number of molecules	3
	Total formula weight	1759.6
Non-Polymers*	Number of molecules	9
	Total formula weight	1642.7
All*	Total formula weight	466096.4

*Water molecules are not included.