8SQH
SpRY-Cas9:gRNA complex targeting TTC PAM DNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | CRISPR-associated endonuclease Cas9/Csn1 | polymer | 1369 | 159149.4 | 1 | UniProt (Q99ZW2) Pfam (PF16593) Pfam (PF16592) Pfam (PF13395) Pfam (PF22702) Pfam (PF16595) | Streptococcus pyogenes | SpCas9,SpyCas9 |
| 2 | B (B) | gRNA | polymer | 98 | 31702.9 | 1 | Streptococcus pyogenes | ||
| 3 | C (C) | TS | polymer | 13 | 4006.6 | 1 | Escherichia phage Lambda | ||
| 4 | D (D) | NTS | polymer | 55 | 16961.9 | 1 | Escherichia phage Lambda | ||
| 5 | E (c) | TS | polymer | 19 | 5761.7 | 1 | Escherichia phage Lambda | ||
| 6 | F, G, H, I (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) |
Sequence modifications
A: 2 - 1368 (UniProt: Q99ZW2)
| PDB | External Database | Details |
|---|---|---|
| Glu 1 | - | expression tag |
| Arg 61 | Ala 61 | engineered mutation |
| Arg 1111 | Leu 1111 | engineered mutation |
| Leu 1135 | Asp 1135 | engineered mutation |
| Trp 1136 | Ser 1136 | engineered mutation |
| Lys 1218 | Gly 1218 | engineered mutation |
| Gln 1219 | Glu 1219 | engineered mutation |
| Arg 1317 | Asn 1317 | engineered mutation |
| Arg 1322 | Ala 1322 | engineered mutation |
| Pro 1333 | Arg 1333 | engineered mutation |
| Gln 1335 | Arg 1335 | engineered mutation |
| Arg 1337 | Thr 1337 | engineered mutation |
| Gly 1369 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 217582.6 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 97.2 | |
| All* | Total formula weight | 217679.8 |
