8SOJ

Cryo-EM structure of human CST bound to POT1(ESDL)/TPP1 in the absence of telomeric ssDNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	CST complex subunit CTC1	polymer	1613	178300.6	1	UniProt (P0AEY0) UniProt (Q2NKJ3) Pfam (PF01547) Pfam (PF15489) UniProt (by SIFTS) (P0AEX9)	Escherichia coli O157:H7	MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP,Conserved telomere maintenance component 1,HBV DNAPTP1-transactivated protein B
2	B (B)	CST complex subunit STN1	polymer	1049	115627.2	1	UniProt (B6F2F5) UniProt (Q96AP0) UniProt (Q9H668) Pfam (PF01353) Pfam (PF10341) Pfam (PF01336) Pfam (PF09170)	Human enterovirus 71 (EV71 EV-71)	POT1 and TIN2-interacting protein,Oligonucleotide/oligosaccharide-binding fold-containing protein 1,Suppressor of cdc thirteen homolog
3	C (C)	CST complex subunit TEN1	polymer	123	13872.0	1	UniProt (Q86WV5) Pfam (PF15490)	Homo sapiens (human)	Protein telomeric pathways with STN1 homolog,Telomere length regulation protein TEN1 homolog
4	D (D)	Protection of telomeres protein 1	polymer	646	72938.2	1	UniProt (Q9NUX5) Pfam (PF21375) Pfam (PF02765) Pfam (PF16686)	Homo sapiens (human)	hPot1,POT1-like telomere end-binding protein
5	E, F (A, D)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Sequence modifications

A: -381 - -16 (UniProt: P0AEY0)

PDB	External Database	Details
Met -395	-	initiating methionine
Gly -394	-	expression tag
Ser -393	-	expression tag
Ser -392	-	expression tag
His -391	-	expression tag
His -390	-	expression tag
His -389	-	expression tag
His -388	-	expression tag
His -387	-	expression tag
His -386	-	expression tag
Ser -385	-	expression tag
Ser -384	-	expression tag
Gly -383	-	expression tag
Thr -382	-	expression tag
Lys -15	-	linker
Leu -14	-	linker
Val -13	-	linker
Glu -12	-	linker
Lys -11	-	linker
Tyr -10	-	linker
Leu -9	-	linker
Glu -8	-	linker
Val -7	-	linker
Leu -6	-	linker
Phe -5	-	linker
Gln -4	-	linker
Gly -3	-	linker
Pro -2	-	linker
Gly -1	-	linker
Ser 0	-	linker

A: 1 - 1217 (UniProt: Q2NKJ3)
B: -248 - -9 (UniProt: B6F2F5)

PDB	External Database	Details
Met -277	-	initiating methionine
Trp -276	-	expression tag
Ser -275	-	expression tag
His -274	-	expression tag
Pro -273	-	expression tag
Gln -272	-	expression tag
Phe -271	-	expression tag
Glu -270	-	expression tag
Lys -269	-	expression tag
Gly -268	-	expression tag
Gly -267	-	expression tag
Gly -266	-	expression tag
Ser -265	-	expression tag
Gly -264	-	expression tag
Gly -263	-	expression tag
Gly -262	-	expression tag
Ser -261	-	expression tag
Gly -260	-	expression tag
Gly -259	-	expression tag
Ser -258	-	expression tag
Ala -257	-	expression tag
Trp -256	-	expression tag
Ser -255	-	expression tag
His -254	-	expression tag
Pro -253	-	expression tag
Gln -252	-	expression tag
Phe -251	-	expression tag
Glu -250	-	expression tag
Lys -249	-	expression tag
Glu -8	-	linker
Asn -7	-	linker
Leu -6	-	linker
Tyr -5	-	linker
Phe -4	-	linker
Gln -3	-	linker
Gly -2	-	linker
Gly -1	-	linker
Ser 0	-	linker

B: 1 - 397 (UniProt: Q96AP0)

PDB	External Database	Details
Gly 398	-	linker
Gly 399	-	linker
Ser 400	-	linker
Gly 401	-	linker
Gly 402	-	linker
Ser 403	-	linker

B: 404 - 771 (UniProt: Q9H668)
D: 2 - 634 (UniProt: Q9NUX5)

PDB	External Database	Details
Met -7	-	initiating methionine
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag
Glu 320	-	insertion
Ser 320	-	insertion
Asp 320	-	insertion
Leu 320	-	insertion

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	380738.1
Non-Polymers*	Number of molecules	2
	Total formula weight	130.8
All*	Total formula weight	380868.9

*Water molecules are not included.