8SMV

GPR161 Gs heterotrimer

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	R	G-protein coupled receptor 161	polymer	540	59869.9	1	UniProt (Q8N6U8) Pfam (PF00001) In PDB	Homo sapiens (human)	G-protein coupled receptor RE2
2	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (human)	G gamma-I
3	N	Nanobody 35	polymer	142	15398.1	1		Lama glama (llama)
4	A	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	261	30137.0	1	UniProt (P63092) Pfam (PF00503) In PDB	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein
5	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	370	40857.6	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)
6	R	CHOLESTEROL	non-polymer		386.7	1	Chemie (CLR)

Sequence modifications

R: 1 - 529 (UniProt: Q8N6U8)

PDB	External Database	Details
Asp -10	-	expression tag
Tyr -9	-	expression tag
Lys -8	-	expression tag
Asp -7	-	expression tag
Asp -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Ala -3	-	expression tag
Ser -2	-	expression tag
Ile -1	-	expression tag
Asp 0	-	expression tag

A: 5 - 64 (UniProt: P63092)

PDB	External Database	Details
Gly -7	-	expression tag
Gly -6	-	expression tag
Ser -5	-	expression tag
Leu -4	-	expression tag
Glu -3	-	expression tag
Val -2	-	expression tag
Leu -1	-	expression tag
Phe 0	-	expression tag
Gln 1	-	expression tag
Gly 2	-	expression tag
Pro 3	-	expression tag
Ser 4	-	expression tag
Asp 49	Gly 49	conflict
Asn 50	Glu 50	conflict

A: 204 - 394 (UniProt: P63092)

PDB	External Database	Details
Gly 196	-	linker
Gly 197	-	linker
Ser 198	-	linker
Gly 199	-	linker
Gly 200	-	linker
Ser 201	-	linker
Gly 202	-	linker
Gly 203	-	linker
Asp 249	Ala 249	conflict
Asp 252	Ser 252	conflict
-	Asn 254	deletion
-	Met 255	deletion
-	Val 256	deletion
-	Ile 257	deletion
-	Arg 258	deletion
-	Glu 259	deletion
-	Asp 260	deletion
-	Asn 261	deletion
-	Gln 262	deletion
-	Thr 263	deletion
Ala 372	Ile 372	conflict
Ile 375	Val 375	conflict

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -29	-	initiating methionine
His -28	-	expression tag
His -27	-	expression tag
His -26	-	expression tag
His -25	-	expression tag
His -24	-	expression tag
His -23	-	expression tag
Leu -22	-	expression tag
Glu -21	-	expression tag
Val -20	-	expression tag
Leu -19	-	expression tag
Phe -18	-	expression tag
Gln -17	-	expression tag
Gly -16	-	expression tag
Pro -15	-	expression tag
Glu -14	-	expression tag
Asp -13	-	expression tag
Gln -12	-	expression tag
Val -11	-	expression tag
Asp -10	-	expression tag
Pro -9	-	expression tag
Arg -8	-	expression tag
Leu -7	-	expression tag
Ile -6	-	expression tag
Asp -5	-	expression tag
Gly -4	-	expression tag
Lys -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gln 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	154123.8
Non-Polymers*	Number of molecules	1
	Total formula weight	386.7
All*	Total formula weight	154510.5

*Water molecules are not included.