8SL1

Cryo-EM structure of PAPP-A2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Pappalysin-2	polymer	1570	174259.8	1	UniProt (Q9BXP8) Pfam (PF13385) Pfam (PF05572) Pfam (PF00084) In PDB	Homo sapiens (human)	Pregnancy-associated plasma protein A2,PAPP-A2,Pregnancy-associated plasma protein E1,PAPP-E
2	B	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
3	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
4	A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
5	A	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
6		water	water		18.0	1	Chemie (HOH)

Sequence modifications

A: 1 - 1557 (UniProt: Q9BXP8)

PDB	External Database	Details
Gln 500	Glu 734	conflict
Thr 1558	-	expression tag
Arg 1559	-	expression tag
Thr 1560	-	expression tag
Gly 1561	-	expression tag
Gly 1562	-	expression tag
Asp 1563	-	expression tag
Tyr 1564	-	expression tag
Lys 1565	-	expression tag
Asp 1566	-	expression tag
Asp 1567	-	expression tag
Asp 1568	-	expression tag
Asp 1569	-	expression tag
Lys 1570	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	174259.8
Branched	Number of molecules	1
	Total formula weight	424.4
Non-Polymers*	Number of molecules	4
	Total formula weight	366.8
All*	Total formula weight	175050.9

*Water molecules are not included.