8SK7
Cryo-EM structure of designed Influenza HA binder, HA_20, bound to Influenza HA (Strain: Iowa43)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, D, G (A, B, C) | Hemagglutinin HA1 chain | polymer | 321 | 35923.3 | 3 | UniProt (A4GCK8) | Influenza A virus | |
| 2 | B, E, H (G, H, I) | Hemagglutinin | polymer | 232 | 26623.5 | 3 | UniProt (A4GCK8) | Influenza A virus | |
| 3 | C, F, I (X, Y, Z) | HA_20 minibinder (RFdiffusion-designed) | polymer | 65 | 7391.8 | 3 | synthetic construct | ||
| 4 | J, L, N (D, F, K) | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 3 | In PDB GlyTouCan (G15407YE) | |||
| 5 | K, M, O (E, J, L) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 3 | In PDB GlyTouCan (G42666HT) | |||
| 6 | AA, P, Q, R, S... (C, A, B) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 12 | Chemie (NAG) |
Sequence modifications
A, B, C: 11 - 331 (UniProt: A4GCK8)
G, H, I: -4 - 175 (UniProt: A4GCK8)
| PDB | External Database | Details |
|---|---|---|
| Phe 101 | Tyr 108 | conflict |
| Ile 307 | Val 314 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Trp 26 | His 369 | conflict |
| Ile 51 | Lys 394 | conflict |
| Ile 103 | Glu 446 | conflict |
| Ser 176 | - | expression tag |
| Gly 177 | - | expression tag |
| Tyr 178 | - | expression tag |
| Ile 179 | - | expression tag |
| Pro 180 | - | expression tag |
| Glu 181 | - | expression tag |
| Ala 182 | - | expression tag |
| Pro 183 | - | expression tag |
| Arg 184 | - | expression tag |
| Asp 185 | - | expression tag |
| Gly 186 | - | expression tag |
| Gln 187 | - | expression tag |
| Ala 188 | - | expression tag |
| Tyr 189 | - | expression tag |
| Val 190 | - | expression tag |
| Arg 191 | - | expression tag |
| Lys 192 | - | expression tag |
| Asp 193 | - | expression tag |
| Gly 194 | - | expression tag |
| Glu 195 | - | expression tag |
| Trp 196 | - | expression tag |
| Val 197 | - | expression tag |
| Leu 198 | - | expression tag |
| Leu 199 | - | expression tag |
| Ser 200 | - | expression tag |
| Thr 201 | - | expression tag |
| Phe 202 | - | expression tag |
| Leu 203 | - | expression tag |
| Gly 204 | - | expression tag |
| Ser 205 | - | expression tag |
| Gly 206 | - | expression tag |
| Leu 207 | - | expression tag |
| Asn 208 | - | expression tag |
| Asp 209 | - | expression tag |
| Ile 210 | - | expression tag |
| Phe 211 | - | expression tag |
| Glu 212 | - | expression tag |
| Ala 213 | - | expression tag |
| Gln 214 | - | expression tag |
| Lys 215 | - | expression tag |
| Ile 216 | - | expression tag |
| Glu 217 | - | expression tag |
| Trp 218 | - | expression tag |
| His 219 | - | expression tag |
| Glu 220 | - | expression tag |
| Gly 221 | - | expression tag |
| His 222 | - | expression tag |
| His 223 | - | expression tag |
| His 224 | - | expression tag |
| His 225 | - | expression tag |
| His 226 | - | expression tag |
| His 227 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 9 |
| Total formula weight | 209816.0 | |
| Branched | Number of molecules | 6 |
| Total formula weight | 3032.8 | |
| Non-Polymers* | Number of molecules | 12 |
| Total formula weight | 2654.5 | |
| All* | Total formula weight | 215503.3 |
