8SID

Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus dehydroepiandrosterone sulfate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Gamma-aminobutyric acid receptor subunit beta-2	polymer	364	41810.1	2	UniProt (P47870) Pfam (PF02931) Pfam (PF02932)	Homo sapiens (human)	GABA(A) receptor subunit beta-2
2	B, D (B, D)	Gamma-aminobutyric acid receptor subunit alpha-1	polymer	358	41061.2	2	UniProt (P14867) Pfam (PF02931) Pfam (PF02932)	Homo sapiens (human)	GABA(A) receptor subunit alpha-1
3	E (E)	Gamma-aminobutyric acid receptor subunit gamma-2	polymer	417	47673.1	1	UniProt (P18507) Pfam (PF02931) Pfam (PF02932)	Homo sapiens (human)	GABA(A) receptor subunit gamma-2
4	F, H (I, L)	Kappa Fab Light Chain	polymer	213	23505.9	2		Mus musculus (mouse)
5	G, I (J, K)	IgG2b Fab Heavy Chain	polymer	454	49811.0	2		Mus musculus (mouse)
6	J, L, M (F, H, M)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	3	In PDB GlyTouCan (G15407YE)
7	K (G)	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1721.5	1	In PDB GlyTouCan (G40702WU)
8	N (N)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
9	O, U (A, C)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)
10	P, V (A, C)	GAMMA-AMINO-BUTANOIC ACID	non-polymer		103.1	2	Chemie (ABU) PubChem (119)
11	Q (A)	17-oxoandrost-5-en-3beta-yl hydrogen sulfate	non-polymer		368.5	1	Chemie (ZWY) PubChem (12594) PubChem (83891) PubChem (6604099) PubChem (13474726) PubChem (24867494) PubChem (50989979) PubChem (5244) PubChem (135337) PubChem (9799421) PubChem (10317945) PubChem (71752218) PubChem (73759687) PubChem (87399275) PubChem (122130634) PubChem (124318506) PubChem (124784292) PubChem (124784293) PubChem (124910878) PubChem (126969889) PubChem (133126796) PubChem (162910008) PubChem (165431259)
12	AA, R, S, T, Y... (D, B)	PHOSPHATIDYLETHANOLAMINE	non-polymer		734.0	6	Chemie (PTY) PubChem (446872) PubChem (3330137)
13	W (C)	O-[(R)-[(2S)-2-(hexadecanoyloxy)-3-(octadecanoyloxy)propoxy](hydroxy)phosphoryl]-D-serine	non-polymer		764.0	1	Chemie (Q3G) PubChem (52925997) PubChem (145946117) PubChem (75958913)
14	X (D)	(2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate	non-polymer		760.1	1	Chemie (POV) PubChem (65167) PubChem (5497103) PubChem (6436017) PubChem (6506401) PubChem (10908846) PubChem (168614) PubChem (6070971)

Sequence modifications

A, C: 1 - 307 (UniProt: P47870)
A, C: 315 - 341 (UniProt: P47870)

PDB	External Database	Details
Ser 308	-	linker
Gln 309	-	linker
Pro 310	-	linker
Ala 311	-	linker
Arg 312	-	linker
Ala 313	-	linker
Ala 314	-	linker
Val 342	-	expression tag
Asp 343	-	expression tag
Gly 344	-	expression tag
Ser 345	-	expression tag
Gly 346	-	expression tag
Ala 347	-	expression tag
Thr 348	-	expression tag
Asn 349	-	expression tag
Phe 350	-	expression tag
Ser 351	-	expression tag
Leu 352	-	expression tag
Leu 353	-	expression tag
Lys 354	-	expression tag
Gln 355	-	expression tag
Ala 356	-	expression tag
Gly 357	-	expression tag
Asp 358	-	expression tag
Val 359	-	expression tag
Glu 360	-	expression tag
Glu 361	-	expression tag
Asn 362	-	expression tag
Pro 363	-	expression tag
Gly 364	-	expression tag

B, D: 1 - 312 (UniProt: P14867)
B, D: 320 - 358 (UniProt: P14867)

PDB	External Database	Details
Ser 313	-	linker
Gln 314	-	linker
Pro 315	-	linker
Ala 316	-	linker
Arg 317	-	linker
Ala 318	-	linker
Ala 319	-	linker

E: 1 - 322 (UniProt: P18507)

PDB	External Database	Details
Trp -36	-	expression tag
Ser -35	-	expression tag
His -34	-	expression tag
Pro -33	-	expression tag
Gln -32	-	expression tag
Phe -31	-	expression tag
Glu -30	-	expression tag
Lys -29	-	expression tag
Gly -28	-	expression tag
Gly -27	-	expression tag
Gly -26	-	expression tag
Ser -25	-	expression tag
Gly -24	-	expression tag
Gly -23	-	expression tag
Gly -22	-	expression tag
Ser -21	-	expression tag
Gly -20	-	expression tag
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
Ala -16	-	expression tag
Trp -15	-	expression tag
Ser -14	-	expression tag
His -13	-	expression tag
Pro -12	-	expression tag
Gln -11	-	expression tag
Phe -10	-	expression tag
Glu -9	-	expression tag
Lys -8	-	expression tag
Leu -7	-	expression tag
Glu -6	-	expression tag
Val -5	-	expression tag
Leu -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Pro 0	-	expression tag

E: 329 - 357 (UniProt: P18507)

PDB	External Database	Details
Ser 323	-	linker
Gln 324	-	linker
Pro 325	-	linker
Ala 326	-	linker
Arg 327	-	linker
Ala 328	-	linker
Ser 358	-	expression tag
Arg 359	-	expression tag
Gly 360	-	expression tag
Ser 361	-	expression tag
Gly 362	-	expression tag
Ala 363	-	expression tag
Thr 364	-	expression tag
Asn 365	-	expression tag
Phe 366	-	expression tag
Ser 367	-	expression tag
Leu 368	-	expression tag
Leu 369	-	expression tag
Lys 370	-	expression tag
Gln 371	-	expression tag
Ala 372	-	expression tag
Gly 373	-	expression tag
Asp 374	-	expression tag
Val 375	-	expression tag
Glu 376	-	expression tag
Glu 377	-	expression tag
Asn 378	-	expression tag
Pro 379	-	expression tag
Gly 380	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	360049.7
Branched	Number of molecules	5
	Total formula weight	3905.6
Non-Polymers*	Number of molecules	13
	Total formula weight	6945.5
All*	Total formula weight	370900.7

*Water molecules are not included.