8SGO

Human GABAA receptor alpha1-beta2-gamma2 subtype in complex with GABA plus pregnenolone sulfate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Gamma-aminobutyric acid receptor subunit beta-2	polymer	364	41810.1	2	UniProt (P47870) Pfam (PF02931) Pfam (PF02932)	Homo sapiens (human)	GABA(A) receptor subunit beta-2
2	B, D (B, D)	Gamma-aminobutyric acid receptor subunit alpha-1	polymer	358	41061.2	2	UniProt (P14867) Pfam (PF02931) Pfam (PF02932)	Homo sapiens (human)	GABA(A) receptor subunit alpha-1
3	E (E)	Gamma-aminobutyric acid receptor subunit gamma-2	polymer	417	47673.1	1	UniProt (P18507) Pfam (PF02931) Pfam (PF02932)	Homo sapiens (human)	GABA(A) receptor subunit gamma-2
4	F, H (I, L)	Kappa Fab Light Chain	polymer	213	23505.9	2		Mus musculus (mouse)
5	G, I (J, K)	IgG2b Fab Heavy Chain	polymer	454	49811.0	2		Mus musculus (mouse)
6	J, L, M (F, H, M)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	3	In PDB GlyTouCan (G15407YE)
7	K (G)	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1721.5	1	In PDB GlyTouCan (G40702WU)
8	N (N)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
9	O, T (A, C)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)
10	P, U (A, C)	GAMMA-AMINO-BUTANOIC ACID	non-polymer		103.1	2	Chemie (ABU)
11	Q, R, S, W, X... (B, C, D)	PHOSPHATIDYLETHANOLAMINE	non-polymer		734.0	6	Chemie (PTY)
12	V (C)	O-[(R)-[(2S)-2-(hexadecanoyloxy)-3-(octadecanoyloxy)propoxy](hydroxy)phosphoryl]-D-serine	non-polymer		764.0	1	Chemie (Q3G)
13	Z (E)	Pregnenolone sulfate	non-polymer		396.5	1	Chemie (A8W)

Sequence modifications

A, C: 1 - 307 (UniProt: P47870)
A, C: 315 - 341 (UniProt: P47870)

PDB	External Database	Details
Ser 308	-	linker
Gln 309	-	linker
Pro 310	-	linker
Ala 311	-	linker
Arg 312	-	linker
Ala 313	-	linker
Ala 314	-	linker
Val 342	-	expression tag
Asp 343	-	expression tag
Gly 344	-	expression tag
Ser 345	-	expression tag
Gly 346	-	expression tag
Ala 347	-	expression tag
Thr 348	-	expression tag
Asn 349	-	expression tag
Phe 350	-	expression tag
Ser 351	-	expression tag
Leu 352	-	expression tag
Leu 353	-	expression tag
Lys 354	-	expression tag
Gln 355	-	expression tag
Ala 356	-	expression tag
Gly 357	-	expression tag
Asp 358	-	expression tag
Val 359	-	expression tag
Glu 360	-	expression tag
Glu 361	-	expression tag
Asn 362	-	expression tag
Pro 363	-	expression tag
Gly 364	-	expression tag

B, D: 1 - 312 (UniProt: P14867)
B, D: 320 - 358 (UniProt: P14867)

PDB	External Database	Details
Ser 313	-	linker
Gln 314	-	linker
Pro 315	-	linker
Ala 316	-	linker
Arg 317	-	linker
Ala 318	-	linker
Ala 319	-	linker

E: 1 - 322 (UniProt: P18507)

PDB	External Database	Details
Trp -36	-	expression tag
Ser -35	-	expression tag
His -34	-	expression tag
Pro -33	-	expression tag
Gln -32	-	expression tag
Phe -31	-	expression tag
Glu -30	-	expression tag
Lys -29	-	expression tag
Gly -28	-	expression tag
Gly -27	-	expression tag
Gly -26	-	expression tag
Ser -25	-	expression tag
Gly -24	-	expression tag
Gly -23	-	expression tag
Gly -22	-	expression tag
Ser -21	-	expression tag
Gly -20	-	expression tag
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
Ala -16	-	expression tag
Trp -15	-	expression tag
Ser -14	-	expression tag
His -13	-	expression tag
Pro -12	-	expression tag
Gln -11	-	expression tag
Phe -10	-	expression tag
Glu -9	-	expression tag
Lys -8	-	expression tag
Leu -7	-	expression tag
Glu -6	-	expression tag
Val -5	-	expression tag
Leu -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Pro 0	-	expression tag

E: 329 - 357 (UniProt: P18507)

PDB	External Database	Details
Ser 323	-	linker
Gln 324	-	linker
Pro 325	-	linker
Ala 326	-	linker
Arg 327	-	linker
Ala 328	-	linker
Ser 358	-	expression tag
Arg 359	-	expression tag
Gly 360	-	expression tag
Ser 361	-	expression tag
Gly 362	-	expression tag
Ala 363	-	expression tag
Thr 364	-	expression tag
Asn 365	-	expression tag
Phe 366	-	expression tag
Ser 367	-	expression tag
Leu 368	-	expression tag
Leu 369	-	expression tag
Lys 370	-	expression tag
Gln 371	-	expression tag
Ala 372	-	expression tag
Gly 373	-	expression tag
Asp 374	-	expression tag
Val 375	-	expression tag
Glu 376	-	expression tag
Glu 377	-	expression tag
Asn 378	-	expression tag
Pro 379	-	expression tag
Gly 380	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	360049.7
Branched	Number of molecules	5
	Total formula weight	3905.6
Non-Polymers*	Number of molecules	12
	Total formula weight	6213.5
All*	Total formula weight	370168.7

*Water molecules are not included.