8SFS
High Affinity nanobodies against GFP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Green fluorescent protein | polymer | 255 | 28784.4 | 2 | UniProt (P42212) Pfam (PF01353) | Aequorea victoria | |
| 2 | C, D (D, C) | LaG16 | polymer | 148 | 16326.1 | 2 | Lama glama | ||
| 3 | E, F, G, H, I... (A, B, D) | AMMONIUM ION | non-polymer | 18.0 | 11 | Chemie (NH4) | |||
| 4 | AA, BA, CA, DA, HA... (D, C, A) | GLYCEROL | non-polymer | 92.1 | 10 | Chemie (GOL) | |||
| 5 | K, LA, R, S (A, C, B) | CHLORIDE ION | non-polymer | 35.5 | 4 | Chemie (CL) | |||
| 6 | EA, FA, GA, L, V... (C, B, D) | SULFATE ION | non-polymer | 96.1 | 7 | Chemie (SO4) | |||
| 7 | MA, T, U (C, B) | SODIUM ION | non-polymer | 23.0 | 3 | Chemie (NA) | |||
| 8 | NA, OA, PA, QA (A, B, D, C) | water | water | 18.0 | 193 | Chemie (HOH) |
Sequence modifications
A, B: 0 - 238 (UniProt: P42212)
| PDB | External Database | Details |
|---|---|---|
| Ala 1 | - | insertion |
| Cr2 66 | Ser 65 | chromophore |
| Cr2 66 | Tyr 66 | chromophore |
| Cr2 66 | Gly 67 | chromophore |
| Ala 72 | Ser 72 | conflict |
| Gly 239 | - | expression tag |
| Leu 240 | - | expression tag |
| Glu 241 | - | expression tag |
| Val 242 | - | expression tag |
| Leu 243 | - | expression tag |
| Phe 244 | - | expression tag |
| Gln 245 | - | expression tag |
| Gly 246 | - | expression tag |
| Pro 247 | - | expression tag |
| Ser 248 | - | expression tag |
| His 249 | - | expression tag |
| His 250 | - | expression tag |
| His 251 | - | expression tag |
| His 252 | - | expression tag |
| His 253 | - | expression tag |
| His 254 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 90221.1 | |
| Non-Polymers* | Number of molecules | 35 |
| Total formula weight | 2002.6 | |
| All* | Total formula weight | 92223.6 |
