8SFS

High Affinity nanobodies against GFP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-11-17
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0750
Spacegroup name	P 31 2 1
Unit cell lengths	131.772, 131.772, 153.580
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.800 - 2.370
R-factor	0.1909
Rwork	0.190
R-free	0.21830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ema 4krn
RMSD bond length	0.009
RMSD bond angle	0.968
Data reduction software	DENZO
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2-4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.420
High resolution limit [Å]	2.370	5.970	2.370
Rmerge	0.104	0.049	0.919
Total number of observations	785910
Number of reflections	143927	7561	7035
<I/σ(I)>	7.6
Completeness [%]	95.0	99.9	93
Redundancy	5.5	5.8	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.1 M HEPES pH 7.5, 1.26 M ammonium sulphate