8SEA
Cryo-EM structure of a double loaded human UBA7-UBE2L6-ISG15 thioester mimetic complex (Form 1)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Ubiquitin-like modifier-activating enzyme 7 | polymer | 1012 | 111822.1 | 1 | UniProt (P41226) Pfam (PF00899) Pfam (PF16190) Pfam (PF16191) Pfam (PF10585) Pfam (PF09358) | Homo sapiens (human) | Ubiquitin-activating enzyme 7,D8,Ubiquitin-activating enzyme E1 homolog |
| 2 | B, D (B, D) | Ubiquitin-like protein ISG15 | polymer | 157 | 17147.6 | 2 | UniProt (P05161) Pfam (PF00240) | Homo sapiens (human) | Interferon-induced 15 kDa protein,Interferon-induced 17 kDa protein,IP17,Ubiquitin cross-reactive protein,hUCRP |
| 3 | C (C) | Ubiquitin/ISG15-conjugating enzyme E2 L6 | polymer | 152 | 17643.4 | 1 | UniProt (O14933) Pfam (PF00179) | Homo sapiens (human) | E2 ubiquitin-conjugating enzyme L6,Retinoic acid-induced gene B protein,RIG-B,UbcH8,Ubiquitin carrier protein L6,Ubiquitin-protein ligase L6 |
| 4 | E (B) | ADENOSINE MONOPHOSPHATE | non-polymer | 347.2 | 1 | Chemie (AMP) |
Sequence modifications
B, D: 1 - 157 (UniProt: P05161)
C: 2 - 153 (UniProt: O14933)
| PDB | External Database | Details |
|---|---|---|
| Ser 78 | Cys 78 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ser 98 | Cys 98 | engineered mutation |
| Ser 102 | Cys 102 | engineered mutation |
| Lys 121 | Leu 121 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 163760.8 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 347.2 | |
| All* | Total formula weight | 164108.0 |
