8SAI

Cryo-EM structure of GPR34-Gi complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Probable G-protein coupled receptor 34	polymer	466	53316.7	1	UniProt (Q9UPC5) Pfam (PF00001)	Homo sapiens (human)
2	B (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
3	C (D)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40415.0	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
4	D (E)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	356	39141.8	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
5	E (S)	scFv16	polymer	266	28636.8	1		Mus musculus (house mouse)
6	F (A)	O-[(S)-hydroxy{[(2S)-2-hydroxy-3-(octadec-9-enoyloxy)propyl]oxy}phosphoryl]-L-serine	non-polymer		523.6	1	Chemie (S12)

Sequence modifications

A: 38 - 373 (UniProt: Q9UPC5)

PDB	External Database	Details
Met -92	-	initiating methionine
Lys -91	-	expression tag
Thr -90	-	expression tag
Ile -89	-	expression tag
Ile -88	-	expression tag
Ala -87	-	expression tag
Leu -86	-	expression tag
Ser -85	-	expression tag
Tyr -84	-	expression tag
Ile -83	-	expression tag
Phe -82	-	expression tag
Cys -81	-	expression tag
Leu -80	-	expression tag
Val -79	-	expression tag
Phe -78	-	expression tag
Ala -77	-	expression tag
Asp -76	-	expression tag
Tyr -75	-	expression tag
Lys -74	-	expression tag
Asp -73	-	expression tag
Asp -72	-	expression tag
Asp -71	-	expression tag
Asp -70	-	expression tag
Lys -69	-	expression tag
Ala -68	-	expression tag
Asp -67	-	expression tag
Leu -66	-	expression tag
Glu -65	-	expression tag
Asp -64	-	expression tag
Asn -63	-	expression tag
Trp -62	-	expression tag
Glu -61	-	expression tag
Thr -60	-	expression tag
Leu -59	-	expression tag
Asn -58	-	expression tag
Asp -57	-	expression tag
Asn -56	-	expression tag
Leu -55	-	expression tag
Lys -54	-	expression tag
Val -53	-	expression tag
Ile -52	-	expression tag
Glu -51	-	expression tag
Lys -50	-	expression tag
Ala -49	-	expression tag
Asp -48	-	expression tag
Asn -47	-	expression tag
Ala -46	-	expression tag
Ala -45	-	expression tag
Gln -44	-	expression tag
Val -43	-	expression tag
Lys -42	-	expression tag
Asp -41	-	expression tag
Ala -40	-	expression tag
Leu -39	-	expression tag
Thr -38	-	expression tag
Lys -37	-	expression tag
Met -36	-	expression tag
Arg -35	-	expression tag
Ala -34	-	expression tag
Ala -33	-	expression tag
Ala -32	-	expression tag
Leu -31	-	expression tag
Asp -30	-	expression tag
Ala -29	-	expression tag
Gln -28	-	expression tag
Lys -27	-	expression tag
Ala -26	-	expression tag
Thr -25	-	expression tag
Pro -24	-	expression tag
Pro -23	-	expression tag
Lys -22	-	expression tag
Leu -21	-	expression tag
Glu -20	-	expression tag
Asp -19	-	expression tag
Lys -18	-	expression tag
Ser -17	-	expression tag
Pro -16	-	expression tag
Asp -15	-	expression tag
Ser -14	-	expression tag
Pro -13	-	expression tag
Glu -12	-	expression tag
Met -11	-	expression tag
Lys -10	-	expression tag
Asp -9	-	expression tag
Phe -8	-	expression tag
Arg -7	-	expression tag
His -6	-	expression tag
Gly -5	-	expression tag
Phe -4	-	expression tag
Asp -3	-	expression tag
Ile -2	-	expression tag
Leu -1	-	expression tag
Val 0	-	expression tag
Gly 1	-	expression tag
Gln 2	-	expression tag
Ile 3	-	expression tag
Asp 4	-	expression tag
Asp 5	-	expression tag
Ala 6	-	expression tag
Leu 7	-	expression tag
Lys 8	-	expression tag
Leu 9	-	expression tag
Ala 10	-	expression tag
Asn 11	-	expression tag
Glu 12	-	expression tag
Gly 13	-	expression tag
Lys 14	-	expression tag
Val 15	-	expression tag
Lys 16	-	expression tag
Glu 17	-	expression tag
Ala 18	-	expression tag
Gln 19	-	expression tag
Ala 20	-	expression tag
Ala 21	-	expression tag
Ala 22	-	expression tag
Glu 23	-	expression tag
Gln 24	-	expression tag
Leu 25	-	expression tag
Lys 26	-	expression tag
Thr 27	-	expression tag
Thr 28	-	expression tag
Arg 29	-	expression tag
Asn 30	-	expression tag
Ala 31	-	expression tag
Tyr 32	-	expression tag
Ile 33	-	expression tag
Gln 34	-	expression tag
Lys 35	-	expression tag
Tyr 36	-	expression tag
Leu 37	-	expression tag

E: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -15	-	initiating methionine
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	169371.4
Non-Polymers*	Number of molecules	1
	Total formula weight	523.6
All*	Total formula weight	169895.0

*Water molecules are not included.