8S84
KOD-H4 DNA polymerase mutant in a ternary complex with DNA/DNA and non-hydrolyzable triphosphate
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA polymerase | polymer | 774 | 89976.5 | 1 | UniProt (D0VWU9) Pfam (PF03104) Pfam (PF00136) | Thermococcus kodakarensis KOD1 | |
| 2 | B (P) | DNA (5'-D(*GP*AP*CP*CP*AP*CP*GP*GP*CP*CP*AP*CP*A*(XG4))-3') | polymer | 13 | 3930.6 | 1 | synthetic construct | ||
| 3 | C (T) | DNA (5'-D(P*AP*CP*TP*GP*TP*GP*GP*CP*CP*GP*TP*GP*GP*TP*C)-3') | polymer | 16 | 4930.2 | 1 | synthetic construct | ||
| 4 | D, E, F, H, I (A) | MAGNESIUM ION | non-polymer | 24.3 | 5 | Chemie (MG) | |||
| 5 | G (A) | 2'-deoxy-5'-O-[(R)-hydroxy{[(R)-hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl]guanosine | non-polymer | 506.2 | 1 | Chemie (XG4) | |||
| 6 | J (A) | MANGANESE (II) ION | non-polymer | 54.9 | 1 | Chemie (MN) | |||
| 7 | K (A) | IMIDAZOLE | non-polymer | 69.1 | 1 | Chemie (IMD) | |||
| 8 | L, M (P, T) | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
| 9 | N, O (A, T) | water | water | 18.0 | 14 | Chemie (HOH) |
Sequence modifications
A: 1 - 774 (UniProt: D0VWU9)
| PDB | External Database | Details |
|---|---|---|
| Gln 93 | Val 93 | conflict |
| Thr 114 | Ile 114 | conflict |
| Ala 141 | Asp 141 | conflict |
| Ala 143 | Glu 143 | conflict |
| His 147 | Glu 147 | conflict |
| Lys 383 | Ser 383 | conflict |
| Gly 429 | Lys 429 | conflict |
| Leu 445 | Phe 445 | conflict |
| Leu 485 | Ala 485 | conflict |
| Val 493 | Tyr 493 | conflict |
| His 496 | Tyr 496 | conflict |
| Met 497 | Tyr 497 | conflict |
| Phe 499 | Tyr 499 | conflict |
| Glu 500 | Ala 500 | conflict |
| Asn 501 | Arg 501 | conflict |
| Leu 521 | Ile 521 | conflict |
| Lys 584 | Glu 584 | conflict |
| Lys 664 | Glu 664 | conflict |
| Arg 726 | Lys 726 | conflict |
| Lys 735 | Asn 735 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 98837.3 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 935.9 | |
| All* | Total formula weight | 99773.2 |
