8S7G

Cryo-EM structure of Pseudomonas aeruginosa Recombinase A (RecA) in complex with LexAS125A mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	LexA repressor	polymer	211	23407.9	2	UniProt (P37452) Pfam (PF01726) Pfam (PF00717)	Pseudomonas aeruginosa
2	C, D, E, F, G... (D, E, F, G, H...)	Protein RecA	polymer	361	38845.2	11	UniProt (P08280) Pfam (PF00154) Pfam (PF21096)	Pseudomonas aeruginosa	Recombinase A
3	L (T)	DNA (36-MER)	polymer	36	10906.0	1		synthetic construct
4	AA, CA, EA, GA, IA... (J, K, L, C, M...)	MAGNESIUM ION	non-polymer		24.3	11	Chemie (MG)
5	BA, DA, FA, HA, JA... (J, K, L, C, M...)	PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER	non-polymer		523.2	11	Chemie (AGS)

Sequence modifications

A, B: 2 - 204 (UniProt: P37452)

PDB	External Database	Details
Met -6	-	initiating methionine
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Gly 1	-	expression tag
Ala 125	Ser 125	engineered mutation

D, E, F, G, H, I, J, K, L, C, M: 2 - 346 (UniProt: P08280)

PDB	External Database	Details
Met -14	-	initiating methionine
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
Lys -7	-	expression tag
Leu -6	-	expression tag
Glu -5	-	expression tag
Asn -4	-	expression tag
Leu -3	-	expression tag
Tyr -2	-	expression tag
Phe -1	-	expression tag
Gln 0	-	expression tag
Gly 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	14
	Total formula weight	485018.8
Non-Polymers*	Number of molecules	22
	Total formula weight	6023.1
All*	Total formula weight	491041.8

*Water molecules are not included.