8S7G

Cryo-EM structure of Pseudomonas aeruginosa Recombinase A (RecA) in complex with LexAS125A mutant

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0001217	molecular_function	DNA-binding transcription repressor activity
A	0003677	molecular_function	DNA binding
A	0004252	molecular_function	serine-type endopeptidase activity
A	0006260	biological_process	DNA replication
A	0006281	biological_process	DNA repair
A	0006355	biological_process	regulation of DNA-templated transcription
A	0006508	biological_process	proteolysis
A	0006974	biological_process	DNA damage response
A	0009432	biological_process	SOS response
A	0016787	molecular_function	hydrolase activity
A	0032993	cellular_component	protein-DNA complex
A	0043565	molecular_function	sequence-specific DNA binding
A	0045892	biological_process	negative regulation of DNA-templated transcription
B	0001217	molecular_function	DNA-binding transcription repressor activity
B	0003677	molecular_function	DNA binding
B	0004252	molecular_function	serine-type endopeptidase activity
B	0006260	biological_process	DNA replication
B	0006281	biological_process	DNA repair
B	0006355	biological_process	regulation of DNA-templated transcription
B	0006508	biological_process	proteolysis
B	0006974	biological_process	DNA damage response
B	0009432	biological_process	SOS response
B	0016787	molecular_function	hydrolase activity
B	0032993	cellular_component	protein-DNA complex
B	0043565	molecular_function	sequence-specific DNA binding
B	0045892	biological_process	negative regulation of DNA-templated transcription
C	0000166	molecular_function	nucleotide binding
C	0003677	molecular_function	DNA binding
C	0003684	molecular_function	damaged DNA binding
C	0003697	molecular_function	single-stranded DNA binding
C	0005524	molecular_function	ATP binding
C	0005737	cellular_component	cytoplasm
C	0006259	biological_process	DNA metabolic process
C	0006281	biological_process	DNA repair
C	0006310	biological_process	DNA recombination
C	0006974	biological_process	DNA damage response
C	0008094	molecular_function	ATP-dependent activity, acting on DNA
C	0009432	biological_process	SOS response
C	0140664	molecular_function	ATP-dependent DNA damage sensor activity
D	0000166	molecular_function	nucleotide binding
D	0003677	molecular_function	DNA binding
D	0003684	molecular_function	damaged DNA binding
D	0003697	molecular_function	single-stranded DNA binding
D	0005524	molecular_function	ATP binding
D	0005737	cellular_component	cytoplasm
D	0006259	biological_process	DNA metabolic process
D	0006281	biological_process	DNA repair
D	0006310	biological_process	DNA recombination
D	0006974	biological_process	DNA damage response
D	0008094	molecular_function	ATP-dependent activity, acting on DNA
D	0009432	biological_process	SOS response
D	0140664	molecular_function	ATP-dependent DNA damage sensor activity
E	0000166	molecular_function	nucleotide binding
E	0003677	molecular_function	DNA binding
E	0003684	molecular_function	damaged DNA binding
E	0003697	molecular_function	single-stranded DNA binding
E	0005524	molecular_function	ATP binding
E	0005737	cellular_component	cytoplasm
E	0006259	biological_process	DNA metabolic process
E	0006281	biological_process	DNA repair
E	0006310	biological_process	DNA recombination
E	0006974	biological_process	DNA damage response
E	0008094	molecular_function	ATP-dependent activity, acting on DNA
E	0009432	biological_process	SOS response
E	0140664	molecular_function	ATP-dependent DNA damage sensor activity
F	0000166	molecular_function	nucleotide binding
F	0003677	molecular_function	DNA binding
F	0003684	molecular_function	damaged DNA binding
F	0003697	molecular_function	single-stranded DNA binding
F	0005524	molecular_function	ATP binding
F	0005737	cellular_component	cytoplasm
F	0006259	biological_process	DNA metabolic process
F	0006281	biological_process	DNA repair
F	0006310	biological_process	DNA recombination
F	0006974	biological_process	DNA damage response
F	0008094	molecular_function	ATP-dependent activity, acting on DNA
F	0009432	biological_process	SOS response
F	0140664	molecular_function	ATP-dependent DNA damage sensor activity
G	0000166	molecular_function	nucleotide binding
G	0003677	molecular_function	DNA binding
G	0003684	molecular_function	damaged DNA binding
G	0003697	molecular_function	single-stranded DNA binding
G	0005524	molecular_function	ATP binding
G	0005737	cellular_component	cytoplasm
G	0006259	biological_process	DNA metabolic process
G	0006281	biological_process	DNA repair
G	0006310	biological_process	DNA recombination
G	0006974	biological_process	DNA damage response
G	0008094	molecular_function	ATP-dependent activity, acting on DNA
G	0009432	biological_process	SOS response
G	0140664	molecular_function	ATP-dependent DNA damage sensor activity
H	0000166	molecular_function	nucleotide binding
H	0003677	molecular_function	DNA binding
H	0003684	molecular_function	damaged DNA binding
H	0003697	molecular_function	single-stranded DNA binding
H	0005524	molecular_function	ATP binding
H	0005737	cellular_component	cytoplasm
H	0006259	biological_process	DNA metabolic process
H	0006281	biological_process	DNA repair
H	0006310	biological_process	DNA recombination
H	0006974	biological_process	DNA damage response
H	0008094	molecular_function	ATP-dependent activity, acting on DNA
H	0009432	biological_process	SOS response
H	0140664	molecular_function	ATP-dependent DNA damage sensor activity
I	0000166	molecular_function	nucleotide binding
I	0003677	molecular_function	DNA binding
I	0003684	molecular_function	damaged DNA binding
I	0003697	molecular_function	single-stranded DNA binding
I	0005524	molecular_function	ATP binding
I	0005737	cellular_component	cytoplasm
I	0006259	biological_process	DNA metabolic process
I	0006281	biological_process	DNA repair
I	0006310	biological_process	DNA recombination
I	0006974	biological_process	DNA damage response
I	0008094	molecular_function	ATP-dependent activity, acting on DNA
I	0009432	biological_process	SOS response
I	0140664	molecular_function	ATP-dependent DNA damage sensor activity
J	0000166	molecular_function	nucleotide binding
J	0003677	molecular_function	DNA binding
J	0003684	molecular_function	damaged DNA binding
J	0003697	molecular_function	single-stranded DNA binding
J	0005524	molecular_function	ATP binding
J	0005737	cellular_component	cytoplasm
J	0006259	biological_process	DNA metabolic process
J	0006281	biological_process	DNA repair
J	0006310	biological_process	DNA recombination
J	0006974	biological_process	DNA damage response
J	0008094	molecular_function	ATP-dependent activity, acting on DNA
J	0009432	biological_process	SOS response
J	0140664	molecular_function	ATP-dependent DNA damage sensor activity
K	0000166	molecular_function	nucleotide binding
K	0003677	molecular_function	DNA binding
K	0003684	molecular_function	damaged DNA binding
K	0003697	molecular_function	single-stranded DNA binding
K	0005524	molecular_function	ATP binding
K	0005737	cellular_component	cytoplasm
K	0006259	biological_process	DNA metabolic process
K	0006281	biological_process	DNA repair
K	0006310	biological_process	DNA recombination
K	0006974	biological_process	DNA damage response
K	0008094	molecular_function	ATP-dependent activity, acting on DNA
K	0009432	biological_process	SOS response
K	0140664	molecular_function	ATP-dependent DNA damage sensor activity
L	0000166	molecular_function	nucleotide binding
L	0003677	molecular_function	DNA binding
L	0003684	molecular_function	damaged DNA binding
L	0003697	molecular_function	single-stranded DNA binding
L	0005524	molecular_function	ATP binding
L	0005737	cellular_component	cytoplasm
L	0006259	biological_process	DNA metabolic process
L	0006281	biological_process	DNA repair
L	0006310	biological_process	DNA recombination
L	0006974	biological_process	DNA damage response
L	0008094	molecular_function	ATP-dependent activity, acting on DNA
L	0009432	biological_process	SOS response
L	0140664	molecular_function	ATP-dependent DNA damage sensor activity
M	0000166	molecular_function	nucleotide binding
M	0003677	molecular_function	DNA binding
M	0003684	molecular_function	damaged DNA binding
M	0003697	molecular_function	single-stranded DNA binding
M	0005524	molecular_function	ATP binding
M	0005737	cellular_component	cytoplasm
M	0006259	biological_process	DNA metabolic process
M	0006281	biological_process	DNA repair
M	0006310	biological_process	DNA recombination
M	0006974	biological_process	DNA damage response
M	0008094	molecular_function	ATP-dependent activity, acting on DNA
M	0009432	biological_process	SOS response
M	0140664	molecular_function	ATP-dependent DNA damage sensor activity

Functional Information from PROSITE/UniProt

site_id	PS00321
Number of Residues	9
Details	RECA_1 recA signature. ALKFYASVR

Chain	Residue	Details
D	ALA213-ARG221

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	11
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00268

Chain	Residue	Details
D	GLY65
C	GLY65
M	GLY65
E	GLY65
F	GLY65
G	GLY65
H	GLY65
I	GLY65
J	GLY65
K	GLY65
L	GLY65

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site_id	SWS_FT_FI2
Number of Residues	4
Details	ACT_SITE: For autocatalytic cleavage activity => ECO:0000255|HAMAP-Rule:MF_00015

Chain	Residue	Details
A	ALA125
A	LYS162
B	ALA125
B	LYS162

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site_id	SWS_FT_FI3
Number of Residues	2
Details	SITE: Cleavage; by autolysis => ECO:0000255|HAMAP-Rule:MF_00015

Chain	Residue	Details
A	ALA90
B	ALA90

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