8S24

Structure of the E3 ubiquitin ligase RNF213, determined by cryoEM

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	E3 ubiquitin-protein ligase RNF213	polymer	5247	596451.3	1	UniProt (Q63HN8) Pfam (PF00097) Pfam (PF20173)	Homo sapiens (human)	ALK lymphoma oligomerization partner on chromosome 17,E3 ubiquitin-lipopolysaccharide ligase RNF213,Mysterin,RING finger protein 213
2	B (A)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	1	Chemie (ATP)
3	C (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
4	D (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)

Sequence modifications

A: 1 - 5207 (UniProt: Q63HN8)

PDB	External Database	Details
Met -39	-	initiating methionine
Ala -38	-	expression tag
Ser -37	-	expression tag
Trp -36	-	expression tag
Ser -35	-	expression tag
His -34	-	expression tag
Pro -33	-	expression tag
Gln -32	-	expression tag
Phe -31	-	expression tag
Glu -30	-	expression tag
Lys -29	-	expression tag
Gly -28	-	expression tag
Ser -27	-	expression tag
Ala -26	-	expression tag
Gly -25	-	expression tag
Ser -24	-	expression tag
Ala -23	-	expression tag
Ala -22	-	expression tag
Gly -21	-	expression tag
Ser -20	-	expression tag
Gly -19	-	expression tag
Ala -18	-	expression tag
Gly -17	-	expression tag
Trp -16	-	expression tag
Ser -15	-	expression tag
His -14	-	expression tag
Pro -13	-	expression tag
Gln -12	-	expression tag
Phe -11	-	expression tag
Glu -10	-	expression tag
Lys -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Ala -2	-	expression tag
Met -1	-	expression tag
Ser 0	-	expression tag
Asp 1045	Asn 1045	variant

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	596451.3
Non-Polymers*	Number of molecules	3
	Total formula weight	596.9
All*	Total formula weight	597048.1

*Water molecules are not included.