8S1U

YlmH bound to stalled 50S subunits with RqcH and PtRNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	c, a	P-tRNA	polymer	76	24491.5	2	GenBank (1837880844)	Bacillus subtilis subsp. subtilis str. 168
2	0	50S ribosomal protein L32	polymer	59	6745.1	1	UniProt (O34687) Pfam (PF01783) In PDB	Bacillus subtilis subsp. subtilis str. 168
3	1	50S ribosomal protein L33 1	polymer	49	5915.9	1	UniProt (P56849) Pfam (PF00471) In PDB	Bacillus subtilis subsp. subtilis str. 168
4	2	50S ribosomal protein L34	polymer	44	5271.3	1	UniProt (P05647) Pfam (PF00468) In PDB	Bacillus subtilis subsp. subtilis str. 168
5	3	50S ribosomal protein L35	polymer	66	7581.1	1	UniProt (P55874) Pfam (PF01632) In PDB	Bacillus subtilis subsp. subtilis str. 168
6	4	50S ribosomal protein L36	polymer	37	4318.4	1	UniProt (P20278) Pfam (PF00444) In PDB	Bacillus subtilis subsp. subtilis str. 168	BL38,Ribosomal protein B,Ribosomal protein II
7	6	50S ribosomal protein L31	polymer	66	7458.6	1	UniProt (Q03223) Pfam (PF01197) In PDB	Bacillus subtilis subsp. subtilis str. 168
8	B	5S rRNA	polymer	112	36157.5	1	GenBank (1150402534)	Bacillus subtilis subsp. subtilis str. 168
9	C	50S ribosomal protein L2	polymer	277	30335.1	1	UniProt (P42919) Pfam (PF00181) Pfam (PF03947) In PDB	Bacillus subtilis subsp. subtilis str. 168	BL2
10	D	50S ribosomal protein L3	polymer	209	22723.3	1	UniProt (P42920) Pfam (PF00297) In PDB	Bacillus subtilis subsp. subtilis str. 168	BL3
11	E	50S ribosomal protein L4	polymer	207	22425.0	1	UniProt (P42921) Pfam (PF00573) In PDB	Bacillus subtilis subsp. subtilis str. 168
12	F	50S ribosomal protein L5	polymer	179	20177.6	1	UniProt (P12877) Pfam (PF00281) Pfam (PF00673) In PDB	Bacillus subtilis subsp. subtilis str. 168	BL6
13	G	Large ribosomal subunit protein uL6	polymer	179	19543.4	1	UniProt (P46898) Pfam (PF00347) In PDB	Bacillus subtilis subsp. subtilis str. 168
14	J	50S ribosomal protein L13	polymer	145	16407.1	1	UniProt (P70974) Pfam (PF00572) In PDB	Bacillus subtilis subsp. subtilis str. 168
15	K	50S ribosomal protein L14	polymer	122	13175.3	1	UniProt (P12875) Pfam (PF00238) In PDB	Bacillus subtilis subsp. subtilis str. 168
16	L	50S ribosomal protein L15	polymer	146	15410.7	1	UniProt (P19946) Pfam (PF00828) In PDB	Bacillus subtilis subsp. subtilis str. 168
17	M	50S ribosomal protein L16	polymer	144	16223.0	1	UniProt (P14577) Pfam (PF00252) In PDB	Bacillus subtilis subsp. subtilis str. 168
18	N	50S ribosomal protein L17	polymer	120	13774.8	1	UniProt (P20277) Pfam (PF01196) In PDB	Bacillus subtilis subsp. subtilis str. 168	BL15,BL21
19	O	50S ribosomal protein L18	polymer	120	12993.8	1	UniProt (P46899) Pfam (PF00861) In PDB	Bacillus subtilis subsp. subtilis str. 168	BL16
20	P	50S ribosomal protein L19	polymer	115	13416.9	1	UniProt (O31742) Pfam (PF01245) In PDB	Bacillus subtilis subsp. subtilis str. 168
21	Q	Large ribosomal subunit protein bL20	polymer	119	13669.2	1	UniProt (P55873) Pfam (PF00453) In PDB	Bacillus subtilis subsp. subtilis str. 168
22	R	50S ribosomal protein L21	polymer	102	11296.1	1	UniProt (P26908) Pfam (PF00829) In PDB	Bacillus subtilis subsp. subtilis str. 168	BL20
23	S	50S ribosomal protein L22	polymer	113	12481.6	1	UniProt (P42060) Pfam (PF00237) In PDB	Bacillus subtilis subsp. subtilis str. 168
24	T	Large ribosomal subunit protein uL23	polymer	95	10978.8	1	UniProt (P42924) Pfam (PF00276) In PDB	Bacillus subtilis subsp. subtilis str. 168
25	U	50S ribosomal protein L24	polymer	103	11166.1	1	UniProt (P0CI78) Pfam (PF00467) Pfam (PF17136) In PDB	Bacillus subtilis subsp. subtilis str. 168	12 kDa DNA-binding protein,BL23,HPB12
26	V	Putative RNA-binding protein YlmH	polymer	275	30823.8	1	UniProt (P71020) Pfam (PF21278) Pfam (PF17774) Pfam (PF01479) In PDB	Bacillus subtilis subsp. subtilis str. 168
27	W	Large ribosomal subunit protein bL27	polymer	94	10391.9	1	UniProt (P05657) Pfam (PF01016) In PDB	Bacillus subtilis subsp. subtilis str. 168
28	X	50S ribosomal protein L28	polymer	62	6826.1	1	UniProt (P37807) Pfam (PF00830) In PDB	Bacillus subtilis subsp. subtilis str. 168
29	Y	50S ribosomal protein L29	polymer	66	7728.0	1	UniProt (P12873) Pfam (PF00831) In PDB	Bacillus subtilis subsp. subtilis str. 168
30	Z	Large ribosomal subunit protein uL30	polymer	59	6650.8	1	UniProt (P19947) Pfam (PF00327) In PDB	Bacillus subtilis subsp. subtilis str. 168
31	H	Rqc2 homolog RqcH	polymer	570	65307.4	1	UniProt (O34693) Pfam (PF05833) Pfam (PF05670) In PDB	Bacillus subtilis subsp. subtilis str. 168	RqcH
32	A	23S rRNA	polymer	2928	949761.4	1		Bacillus subtilis subsp. subtilis str. 168
33	A	POTASSIUM ION	non-polymer		39.1	15	Chemie (K)

Sequence modifications

V: 1 - 257 (UniProt: P71020)

PDB	External Database	Details
Gly 258	-	expression tag
Ser 259	-	expression tag
Gly 260	-	expression tag
Ser 261	-	expression tag
Gly 262	-	expression tag
Ser 263	-	expression tag
Gly 264	-	expression tag
Ser 265	-	expression tag
Gly 266	-	expression tag
Ser 267	-	expression tag
Asp 268	-	expression tag
Tyr 269	-	expression tag
Lys 270	-	expression tag
Asp 271	-	expression tag
Asp 272	-	expression tag
Asp 273	-	expression tag
Asp 274	-	expression tag
Lys 275	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	33
	Total formula weight	1476118.3
Non-Polymers*	Number of molecules	15
	Total formula weight	586.5
All*	Total formula weight	1476704.7

*Water molecules are not included.