8S1L

Crystal structure of Renilla reniformis luciferase RLuc8-GFP BRET complex at pH 6.0

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, E, F (A, B, E, F)	Green fluorescent protein	polymer	233	26017.8	4	UniProt (Q963I9) Pfam (PF01353)	Renilla reniformis (sea pansy)
2	C, D, G, H (C, D, G, H)	Coelenterazine h 2-monooxygenase	polymer	319	36992.3	4	UniProt (P27652) Pfam (PF00561)	Renilla reniformis (sea pansy)	Renilla-luciferin 2-monooxygenase,Renilla-type luciferase
3	I, J, K, L (C, D, G, H)	N-[3-BENZYL-5-(4-HYDROXYPHENYL)PYRAZIN-2-YL]-2-(4-HYDROXYPHENYL)ACETAMIDE	non-polymer		411.5	4	Chemie (CEI)
4	M, N, O, P, Q... (A, B, C, D, E...)	water	water		18.0	93	Chemie (HOH)

Sequence modifications

A, B, E, F: 1 - 231 (UniProt: Q963I9)

PDB	External Database	Details
Cro 66	Ser 66	chromophore
Cro 66	Tyr 67	chromophore
Cro 66	Gly 68	chromophore

C, D, G, H: 1 - 311 (UniProt: P27652)

PDB	External Database	Details
Thr 55	Ala 55	engineered mutation
Ala 124	Cys 124	engineered mutation
Ala 130	Ser 130	engineered mutation
Arg 136	Lys 136	engineered mutation
Met 143	Ala 143	engineered mutation
Val 185	Met 185	engineered mutation
Leu 253	Met 253	engineered mutation
Leu 287	Ser 287	engineered mutation
Ser 312	-	expression tag
Gly 313	-	expression tag
Leu 314	-	expression tag
Glu 315	-	expression tag
Val 316	-	expression tag
Leu 317	-	expression tag
Phe 318	-	expression tag
Gln 319	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	252040.2
Non-Polymers*	Number of molecules	4
	Total formula weight	1645.8
All*	Total formula weight	253686.0

*Water molecules are not included.