8S1L

Crystal structure of Renilla reniformis luciferase RLuc8-GFP BRET complex at pH 6.0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-14
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 32
Unit cell lengths	95.934, 95.934, 357.132
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.540 - 2.398
R-factor	0.271
Rwork	0.268
R-free	0.33950
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.487
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.540	2.480
High resolution limit [Å]	2.398	2.398
Number of reflections	143749	14312
<I/σ(I)>	9.9
Completeness [%]	99.0
Redundancy	10.5
CC(1/2)	0.998	0.369

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293.15	MES buffer, PEG 4000