8S0M
Crystal structure of the HKU1 receptor binding domain in complex with TMPRSS2 and the nanobody A01
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | B, E | Transmembrane protease serine 2 | polymer | 395 | 43678.2 | 2 | UniProt (O15393) Pfam (PF15494) Pfam (PF00089) In PDB | Homo sapiens (human) | Serine protease 10 |
2 | U, V | Nanobody A01 | polymer | 136 | 14961.5 | 2 | Vicugna pacos | ||
3 | A, D | Spike protein S1 | polymer | 366 | 40835.7 | 2 | UniProt (Q0ZME7) Pfam (PF09408) Pfam (PF19209) In PDB | Human coronavirus HKU1 (isolate N5) | |
4 | F | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 1 | In PDB GlyTouCan (G42666HT) | |||
5 | B, A, D | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 4 | Chemie (NAG) | |||
6 | A, D | PHOSPHATE ION | non-polymer | 95.0 | 2 | Chemie (PO4) | |||
7 | water | water | 18.0 | 1 | Chemie (HOH) |
Sequence modifications
B, E: 107 - 492 (UniProt: O15393)
A, D: 1 - 366 (UniProt: Q0ZME7)
PDB | External Database | Details |
---|---|---|
Ala 441 | Ser 441 | engineered mutation |
Gly 493 | - | expression tag |
Pro 494 | - | expression tag |
Phe 495 | - | expression tag |
Glu 496 | - | expression tag |
Asp 497 | - | expression tag |
Asp 498 | - | expression tag |
Asp 499 | - | expression tag |
Asp 500 | - | expression tag |
Lys 501 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Val 333 | Ala 639 | conflict |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 6 |
Total formula weight | 198950.7 | |
Branched | Number of molecules | 1 |
Total formula weight | 424.4 | |
Non-Polymers* | Number of molecules | 6 |
Total formula weight | 1074.8 | |
All* | Total formula weight | 200449.9 |