8RZK

The Michaelis complex of ZgGH129 D486N from Zobellia galactanivorans with neo-b/k-oligo-carrageenan tetrasaccharide (beta-kappa neo-oligo-carrageenan DP4).

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Conserved hypothetical periplasmic protein	polymer	676	76227.1	4	UniProt (G0L004) Pfam (PF18952)	Zobellia galactanivorans
2	E, F, G, H (E, F, G, H)	3,6-anhydro-alpha-D-galactopyranose-(1-3)-beta-D-galactopyranose-(1-4)-3,6-anhydro-alpha-D-galactopyranose-(1-3)-4-O-sulfo-beta-D-galactopyranose	branched		710.6	4	In PDB GlyTouCan (G69730VZ)
3	I, J, M, Q, T (A, B, C, D)	L(+)-TARTARIC ACID	non-polymer		150.1	5	Chemie (TLA)
4	K, O, R, U (A, B, C, D)	SODIUM ION	non-polymer		23.0	4	Chemie (NA)
5	L, P, S, V (A, B, C, D)	CHLORIDE ION	non-polymer		35.5	4	Chemie (CL)
6	N (B)	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
7	W, X, Y, Z (A, B, C, D)	water	water		18.0	1146	Chemie (HOH)

Sequence modifications

A, B, C, D: 32 - 693 (UniProt: G0L004)

PDB	External Database	Details
Met 18	-	initiating methionine
Gly 19	-	expression tag
Ser 20	-	expression tag
Ser 21	-	expression tag
His 22	-	expression tag
His 23	-	expression tag
His 24	-	expression tag
His 25	-	expression tag
His 26	-	expression tag
His 27	-	expression tag
Gly 28	-	expression tag
Ser 29	-	expression tag
Leu 30	-	expression tag
Asp 31	-	expression tag
Asn 486	Asp 486	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	304908.5
Branched	Number of molecules	4
	Total formula weight	2842.4
Non-Polymers*	Number of molecules	14
	Total formula weight	1046.3
All*	Total formula weight	308797.2

*Water molecules are not included.