8RZK

The Michaelis complex of ZgGH129 D486N from Zobellia galactanivorans with neo-b/k-oligo-carrageenan tetrasaccharide (beta-kappa neo-oligo-carrageenan DP4).

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9801
Spacegroup name	I 1 2 1
Unit cell lengths	162.734, 105.943, 213.664
Unit cell angles	90.00, 111.23, 90.00

Refinement procedure

Resolution	47.820 - 2.290
R-factor	0.1705
Rwork	0.169
R-free	0.20160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.810
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.890	2.330
High resolution limit [Å]	2.290	2.290
Rmeas	0.161	1.500
Number of reflections	152074	7468
<I/σ(I)>	9.2	1.5
Completeness [%]	100.0	100
Redundancy	6.9	7.1
CC(1/2)	0.996	0.551

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.63 mg/ml 2:1 ratio 0.14M Na/K-tartrate, 14% PEG 3350