8RZI

ZgGH129 from Zobellia galactanivorans soaked with 1,2-diF-ADG (3,6-Anhydro-2-deoxy-2-fluoro-a-D-galactopyranosyl fluoride) resulting in a trapped glycosyl-enzyme intermediate.

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Conserved hypothetical periplasmic protein	polymer	676	76228.1	4	UniProt (G0L004) Pfam (PF18952)	Zobellia galactanivorans
2	E, KA, P, Z (A, D, B, C)	(1~{R},4~{S},5~{S},8~{S})-4-fluoranyl-2,6-dioxabicyclo[3.2.1]octan-8-ol	non-polymer		148.1	4	Chemie (A1H37)
3	AA, F, LA, Q (C, A, D, B)	L(+)-TARTARIC ACID	non-polymer		150.1	4	Chemie (TLA)
4	G, R (A, B)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
5	BA, H, MA, S (C, A, D, B)	SODIUM ION	non-polymer		23.0	4	Chemie (NA)
6	CA, DA, I, J, NA... (C, A, D, B)	CHLORIDE ION	non-polymer		35.5	8	Chemie (CL)
7	EA, FA, GA, HA, IA... (C, A, D, B)	1,2-ETHANEDIOL	non-polymer		62.1	23	Chemie (EDO)
8	AB, XA, YA, ZA (D, A, B, C)	water	water		18.0	2249	Chemie (HOH)

Sequence modifications

A, B, C, D: 32 - 693 (UniProt: G0L004)

PDB	External Database	Details
Met 18	-	initiating methionine
Gly 19	-	expression tag
Ser 20	-	expression tag
Ser 21	-	expression tag
His 22	-	expression tag
His 23	-	expression tag
His 24	-	expression tag
His 25	-	expression tag
His 26	-	expression tag
His 27	-	expression tag
Gly 28	-	expression tag
Ser 29	-	expression tag
Leu 30	-	expression tag
Asp 31	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	304912.4
Non-Polymers*	Number of molecules	45
	Total formula weight	3208.3
All*	Total formula weight	308120.7

*Water molecules are not included.