8RWV

Human OCCM DNA licensing intermediate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (2)	DNA replication licensing factor MCM2	polymer	947	106435.8	1	UniProt (P49736) Pfam (PF12619) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF23669)	Homo sapiens (human)	Minichromosome maintenance protein 2 homolog,Nuclear protein BM28
2	B (3)	Isoform 2 of DNA replication licensing factor MCM3	polymer	853	96043.3	1	UniProt (P25205) Pfam (PF17855) Pfam (PF23191) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493)	Homo sapiens (human)	DNA polymerase alpha holoenzyme-associated protein P1,P1-MCM3,RLF subunit beta,p102
3	C (4)	DNA replication licensing factor MCM4	polymer	883	99119.5	1	UniProt (P33991) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF21128)	Homo sapiens (human)	CDC21 homolog,P1-CDC21
4	D (6)	DNA replication licensing factor MCM6	polymer	821	93010.3	1	UniProt (Q14566) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF18263)	Homo sapiens (human)	p105MCM
5	E (7)	DNA replication licensing factor MCM7	polymer	719	81411.9	1	UniProt (P33993) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF24901)	Homo sapiens (human)	CDC47 homolog,P1.1-MCM3
6	F (A)	Origin recognition complex subunit 1	polymer	961	108813.6	1	UniProt (Q13415) Pfam (PF01426) Pfam (PF00004) Pfam (PF17872) Pfam (PF09079)	Homo sapiens	Replication control protein 1
7	G (B)	Origin recognition complex subunit 2	polymer	577	66063.4	1	UniProt (Q13416) Pfam (PF04084) Pfam (PF24882)	Homo sapiens (human)
8	H (C)	Isoform 2 of Origin recognition complex subunit 3	polymer	712	82436.1	1	UniProt (Q9UBD5) Pfam (PF07034) Pfam (PF19675) Pfam (PF18137)	Homo sapiens (human)	Origin recognition complex subunit Latheo
9	I (D)	Origin recognition complex subunit 4	polymer	436	50443.3	1	UniProt (O43929) Pfam (PF13191) Pfam (PF14629)	Homo sapiens (human)
10	J (E)	Origin recognition complex subunit 5	polymer	435	50349.9	1	UniProt (O43913) Pfam (PF13191) Pfam (PF21639) Pfam (PF14630)	Homo sapiens (human)
11	K (F)	Cell division control protein 6 homolog	polymer	560	62834.4	1	UniProt (Q99741) Pfam (PF13191) Pfam (PF22606) Pfam (PF09079)	Homo sapiens (human)	CDC6-related protein,Cdc18-related protein,HsCdc18,p62(cdc6),HsCDC6
12	L (G)	DNA replication factor Cdt1	polymer	410	46099.0	1	UniProt (Q9H211) Pfam (PF08839) Pfam (PF16679)	Homo sapiens (human)	Double parked homolog,DUP
13	M (H)	DNA	polymer	39	11933.7	1		Homo sapiens
14	N (J)	DNA	polymer	40	12380.0	1		Homo sapiens

Sequence modifications

2: 1 - 904 (UniProt: P49736)

PDB	External Database	Details
Met -42	-	initiating methionine
Ser -41	-	expression tag
Ala -40	-	expression tag
Trp -39	-	expression tag
Ser -38	-	expression tag
His -37	-	expression tag
Pro -36	-	expression tag
Gln -35	-	expression tag
Phe -34	-	expression tag
Glu -33	-	expression tag
Lys -32	-	expression tag
Gly -31	-	expression tag
Gly -30	-	expression tag
Gly -29	-	expression tag
Ser -28	-	expression tag
Gly -27	-	expression tag
Gly -26	-	expression tag
Gly -25	-	expression tag
Ser -24	-	expression tag
Gly -23	-	expression tag
Gly -22	-	expression tag
Ser -21	-	expression tag
Ala -20	-	expression tag
Trp -19	-	expression tag
Ser -18	-	expression tag
His -17	-	expression tag
Pro -16	-	expression tag
Gln -15	-	expression tag
Phe -14	-	expression tag
Glu -13	-	expression tag
Lys -12	-	expression tag
Glu -11	-	expression tag
Asn -10	-	expression tag
Leu -9	-	expression tag
Tyr -8	-	expression tag
Phe -7	-	expression tag
Gln -6	-	expression tag
Gly -5	-	expression tag
Ala -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Ala -1	-	expression tag
Thr 0	-	expression tag

4: 1 - 863 (UniProt: P33991)

PDB	External Database	Details
Met -19	-	initiating methionine
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Glu -10	-	expression tag
Asn -9	-	expression tag
Leu -8	-	expression tag
Tyr -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Ser -3	-	expression tag
Ser -2	-	expression tag
Ala -1	-	expression tag
Thr 0	-	expression tag

A: -99 - 1 (PDB: 8RWV)
A: 2 - 861 (UniProt: Q13415)
F: 1 - 560 (UniProt: Q99741)

PDB	External Database	Details
Ile 441	Val 441	variant

G: 158 - 546 (UniProt: Q9H211)

PDB	External Database	Details
Met 137	-	initiating methionine
Gly 138	-	expression tag
Ser 139	-	expression tag
Ser 140	-	expression tag
His 141	-	expression tag
His 142	-	expression tag
His 143	-	expression tag
His 144	-	expression tag
His 145	-	expression tag
His 146	-	expression tag
Ser 147	-	expression tag
Ser 148	-	expression tag
Gly 149	-	expression tag
Leu 150	-	expression tag
Glu 151	-	expression tag
Val 152	-	expression tag
Leu 153	-	expression tag
Phe 154	-	expression tag
Gln 155	-	expression tag
Gly 156	-	expression tag
Pro 157	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	14
	Total formula weight	967374.1
All*	Total formula weight	967374.1

*Water molecules are not included.