8ROH

Human cohesin SMC3-HD(EQ)/RAD21-N complex - Apo conformation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Structural maintenance of chromosomes protein 3	polymer	478	54448.8	1	UniProt (Q9UQE7) Pfam (PF02463)	Homo sapiens (human)	SMC protein 3,SMC-3,Basement membrane-associated chondroitin proteoglycan,Bamacan,Chondroitin sulfate proteoglycan 6,Chromosome-associated polypeptide,hCAP
2	B (B)	Double-strand-break repair protein rad21 homolog	polymer	110	12617.9	1	UniProt (O60216) Pfam (PF04825)	Homo sapiens (human)	hHR21,Nuclear matrix protein 1,NXP-1,SCC1 homolog
3	C, D, E (A)	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
4	F (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
5	G (A)	water	water		18.0	9	Chemie (HOH)

Sequence modifications

A: 1 - 958 (UniProt: Q9UQE7)
A: 971 - 1217 (UniProt: Q9UQE7)

PDB	External Database	Details
Gly 959	-	linker
Ser 960	-	linker
Gly 961	-	linker
Ser 962	-	linker
Leu 963	-	linker
Val 964	-	linker
Pro 965	-	linker
Arg 966	-	linker
Gly 967	-	linker
Ser 968	-	linker
Gly 969	-	linker
Ser 970	-	linker
Gln 1144	Glu 1144	engineered mutation

B: 1 - 102 (UniProt: O60216)

PDB	External Database	Details
Gly 103	-	expression tag
Ser 104	-	expression tag
Leu 105	-	expression tag
Glu 106	-	expression tag
Val 107	-	expression tag
Leu 108	-	expression tag
Phe 109	-	expression tag
Gln 110	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	67066.7
Non-Polymers*	Number of molecules	4
	Total formula weight	380.3
All*	Total formula weight	67447.0

*Water molecules are not included.