8RO0

Structure of the C. elegans Intron Lariat Spliceosome primed for disassembly (ILS')

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	2	U2 snRNA	polymer	191	56554.5	1	GenBank (6851)	Caenorhabditis elegans
2	5	U5 snRNA	polymer	121	38680.8	1	GenBank (1200267)	Caenorhabditis elegans
3	6	U6 snRNA	polymer	101	32483.4	1	GenBank (6866)	Caenorhabditis elegans
4	A	Pre-mRNA-splicing factor 8 homolog	polymer	2329	272396.2	1	UniProt (P34369) Pfam (PF08082) Pfam (PF08083) Pfam (PF10598) Pfam (PF10597) Pfam (PF10596) Pfam (PF12134) Pfam (PF01398) Pfam (PF08084) In PDB	Caenorhabditis elegans
5	B	U5 small nuclear ribonucleoprotein 200 kDa helicase	polymer	2145	244151.6	1	UniProt (Q9U2G0) Pfam (PF18149) Pfam (PF00270) Pfam (PF00271) Pfam (PF02889) Pfam (PF21188) In PDB	Caenorhabditis elegans	BRR2 homolog
6	C	Tr-type G domain-containing protein	polymer	974	110612.9	1	UniProt (Q23463) Pfam (PF16004) Pfam (PF00009) Pfam (PF03144) Pfam (PF03764) Pfam (PF00679) In PDB	Caenorhabditis elegans
7	D	Protein isy-1	polymer	267	31326.2	1	UniProt (Q20716) Pfam (PF06246) In PDB	Caenorhabditis elegans
8	E	WD_REPEATS_REGION domain-containing protein	polymer	331	36865.6	1	UniProt (Q19211) Pfam (PF00400) In PDB	Caenorhabditis elegans
9	I	Pre-mRNA-splicing factor SYF1	polymer	855	99675.1	1	UniProt (P91175) Pfam (PF13181) In PDB	Caenorhabditis elegans
10	J	TPR_REGION domain-containing protein	polymer	744	88116.0	1	UniProt (O16376) Pfam (PF02184) In PDB	Caenorhabditis elegans
11	K	Pre-mRNA-splicing factor SPF27	polymer	238	27679.9	1	UniProt (Q22417) Pfam (PF05700) In PDB	Caenorhabditis elegans
12	L	Cell division cycle 5-like protein	polymer	755	85843.5	1	UniProt (G5EFC4) Pfam (PF13921) Pfam (PF11831) In PDB	Caenorhabditis elegans
13	M	Pre-mRNA-splicing factor syf-2	polymer	234	27719.0	1	UniProt (Q09385) Pfam (PF08231) In PDB	Caenorhabditis elegans
14	N	Protein BUD31 homolog	polymer	147	17153.9	1	UniProt (P34313) Pfam (PF01125) In PDB	Caenorhabditis elegans	Protein G10 homolog
15	O	Pre-mRNA-splicing factor RBM22	polymer	408	45902.8	1	UniProt (Q22412) Pfam (PF21369) Pfam (PF16131) Pfam (PF00076) In PDB	Caenorhabditis elegans	RNA-binding motif protein 22
16	P	Spliceosome-associated protein CWC15 homolog	polymer	230	26154.8	1	UniProt (O45766) Pfam (PF04889) In PDB	Caenorhabditis elegans
17	PX	GCF C-terminal domain-containing protein	polymer	809	94244.9	1	UniProt (G5ECH1) Pfam (PF15458) Pfam (PF07842) In PDB	Caenorhabditis elegans
18	Q	Intron-binding protein aquarius	polymer	1467	170397.7	1	UniProt (Q9U1Q7) Pfam (PF16399) Pfam (PF21143) Pfam (PF21144) Pfam (PF13086) Pfam (PF13087) In PDB	Caenorhabditis elegans
19	R	Uncharacterized protein T27F2.1	polymer	535	60303.5	1	UniProt (Q22836) Pfam (PF02731) In PDB	Caenorhabditis elegans
20	S	Peptidyl-prolyl cis-trans isomerase	polymer	169	18547.0	1	UniProt (Q18445) Pfam (PF00160) In PDB	Caenorhabditis elegans	PPIase
21	T	WD_REPEATS_REGION domain-containing protein	polymer	494	54766.2	1	UniProt (G5EEL2) Pfam (PF00400) In PDB	Caenorhabditis elegans
22	TF	Septin and tuftelin-interacting protein 1 homolog	polymer	830	94421.5	1	UniProt (Q17784) Pfam (PF12457) Pfam (PF01585) Pfam (PF07842) In PDB	Caenorhabditis elegans	STIP-1
23	W	WD_REPEATS_REGION domain-containing protein	polymer	567	65385.7	1	UniProt (O44729) Pfam (PF00400) In PDB	Caenorhabditis elegans
24	Z	Coiled-coil domain-containing protein 12	polymer	169	19138.5	1	UniProt (Q95XJ1) Pfam (PF08315) In PDB	Caenorhabditis elegans
25	a, h	Small nuclear ribonucleoprotein Sm D3	polymer	136	14836.2	2	UniProt (Q17348) Pfam (PF01423) In PDB	Caenorhabditis elegans	Sm-D3,snRNP core protein D3
26	b, i	Probable small nuclear ribonucleoprotein-associated protein B	polymer	160	16768.6	2	UniProt (P91918) Pfam (PF01423) In PDB	Caenorhabditis elegans	snRNP-B,Sm protein B,Sm-B,SmB
27	c, j	Small nuclear ribonucleoprotein Sm D1	polymer	127	13724.1	2	UniProt (E3LR34) Pfam (PF01423) In PDB	Caenorhabditis elegans	snRNP core protein D1
28	d, k	Probable small nuclear ribonucleoprotein Sm D2	polymer	118	13291.5	2	UniProt (Q18786) Pfam (PF01423) In PDB	Caenorhabditis elegans	Sm-D2,snRNP core protein D2
29	e, l	Probable small nuclear ribonucleoprotein E	polymer	90	10625.3	2	UniProt (Q9XTU6) Pfam (PF01423) In PDB	Caenorhabditis elegans	snRNP-E,Sm protein E,Sm-E,SmE
30	f, m	Probable small nuclear ribonucleoprotein F	polymer	85	9256.5	2	UniProt (P34659) Pfam (PF01423) In PDB	Caenorhabditis elegans	snRNP-F,Sm protein F,Sm-F,SmF
31	g, n	Probable small nuclear ribonucleoprotein G	polymer	77	8756.2	2	UniProt (Q9N4G9) Pfam (PF01423) In PDB	Caenorhabditis elegans	snRNP-G,Sm protein G,Sm-G,SmG
32	o	Probable U2 small nuclear ribonucleoprotein A'	polymer	253	28905.9	1	UniProt (Q9BLB6) Pfam (PF12799) In PDB	Caenorhabditis elegans	U2 snRNP A',Masculinization of germline protein 2,Spliceosome-associated protein mog-2
33	p	RRM domain-containing protein	polymer	217	24881.3	1	UniProt (Q21323) Pfam (PF00076) In PDB	Caenorhabditis elegans
34	q, r, s, t	Pre-mRNA-processing factor 19	polymer	492	53272.6	4	UniProt (Q10051) Pfam (PF04564) Pfam (PF08606) Pfam (PF00400) In PDB	Caenorhabditis elegans	PRP19/PSO4 homolog,RING-type E3 ubiquitin transferase PRP19
35	y	Peptidyl-prolyl cis-trans isomerase E	polymer	331	36469.3	1	UniProt (Q9U2S6) Pfam (PF00076) Pfam (PF00160) In PDB	Caenorhabditis elegans	PPIase E
36	In	Intron lariat RNA	polymer	19	3680.8	1		Caenorhabditis elegans
37	C, 6	MAGNESIUM ION	non-polymer		24.3	7	Chemie (MG)
38	A, J	INOSITOL HEXAKISPHOSPHATE	non-polymer		660.0	2	Chemie (IHP)
39	C	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	1	Chemie (GTP)
40	N, O	ZINC ION	non-polymer		65.4	6	Chemie (ZN)

Sequence modifications

2: 1 - 149 (GenBank: 6851)
2: 150 - 186 (PDB: 8RO0)
2: 187 - 191 (GenBank: 6851)

Contents of the asymmetric unit

Polymers	Number of chains	46
	Total formula weight	2300065.9
Non-Polymers*	Number of molecules	16
	Total formula weight	2405.8
All*	Total formula weight	2302471.7

*Water molecules are not included.