8RML

Structure of heteromeric CALHM2/4 channel in complex with synthetic nanobody SbC4

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, L (A, B, K)	Calcium homeostasis modulator protein 4	polymer	322	35981.7	3	UniProt (Q5JW98) Pfam (PF14798)	Homo sapiens (human)	Protein FAM26D
2	B, M (L, M)	Synthetic nanobody SbC4	polymer	119	12877.4	2		synthetic construct
3	D, E, F, G, H... (C, D, E, F, G...)	Calcium homeostasis modulator protein 2	polymer	331	37099.5	8	UniProt (Q9HA72) Pfam (PF14798)	Homo sapiens (human)	Protein FAM26B

Sequence modifications

A, B, K: 2 - 314 (UniProt: Q5JW98)

PDB	External Database	Details
Met 0	-	initiating methionine
Ser 1	-	expression tag
Ala 315	-	expression tag
Leu 316	-	expression tag
Glu 317	-	expression tag
Val 318	-	expression tag
Leu 319	-	expression tag
Phe 320	-	expression tag
Gln 321	-	expression tag

C, D, E, F, G, H, I, J: 2 - 323 (UniProt: Q9HA72)

PDB	External Database	Details
Met 0	-	initiating methionine
Ser 1	-	expression tag
Ala 324	-	expression tag
Leu 325	-	expression tag
Glu 326	-	expression tag
Val 327	-	expression tag
Leu 328	-	expression tag
Phe 329	-	expression tag
Gln 330	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	13
	Total formula weight	430496.0
All*	Total formula weight	430496.0

*Water molecules are not included.