8RDI

Crystal structure of UDP-galactose 4-epimerase from Pyrococcus horikoshii containing Y145F mutation and with bound NAD and GDP-L-fucose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	SDR family NAD(P)-dependent oxidoreductase	polymer	326	36695.8	4	UniProt (A0A832T930) Pfam (PF01370) UniProt (by SIFTS) (O73960)	Pyrococcus horikoshii
2	E, G, I, K (A, B, C, D)	NICOTINAMIDE-ADENINE-DINUCLEOTIDE	non-polymer		663.4	4	Chemie (NAD)
3	F, H, J, L (A, B, C, D)	GUANOSINE-5'-DIPHOSPHATE-BETA-L-FUCOPYRANOSE	non-polymer		589.3	4	Chemie (GFB)

Sequence modifications

A, B, C, D: 1 - 318 (UniProt: A0A832T930)

PDB	External Database	Details
Phe 145	Tyr 145	engineered mutation
Leu 319	-	expression tag
Glu 320	-	expression tag
His 321	-	expression tag
His 322	-	expression tag
His 323	-	expression tag
His 324	-	expression tag
His 325	-	expression tag
His 326	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	146783.2
Non-Polymers*	Number of molecules	8
	Total formula weight	5011.1
All*	Total formula weight	151794.2

*Water molecules are not included.