8RB0
The crystal structure of DNA-bound human MutSbeta (MSH2_E749A/MSH3) in the canonical mismatch bound conformation with ADP bound in MSH2
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA mismatch repair protein Msh2 | polymer | 934 | 104803.8 | 1 | UniProt (P43246) | Homo sapiens (human) | |
| 2 | B (B) | DNA mismatch repair protein Msh3 | polymer | 918 | 104289.7 | 1 | UniProt (P20585) | Homo sapiens (human) | |
| 3 | C (C) | DNA (5'-D(P*AP*TP*CP*TP*GP*AP*AP*GP*CP*CP*GP*AP*TP*CP*GP*AP*TP*G)-3') | polymer | 24 | 7345.7 | 1 | synthetic construct | ||
| 4 | D (D) | DNA (5'-D(*TP*CP*AP*TP*CP*GP*AP*TP*CP*GP*CP*AP*GP*CP*TP*TP*CP*AP*GP*AP*TP*AP*G)-3') | polymer | 24 | 7369.8 | 1 | synthetic construct | ||
| 5 | E (A) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 1 | Chemie (ADP) | |||
| 6 | F (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 7 | G (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 8 | H, I (A, B) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 2 | Chemie (EDO) | |||
| 9 | J, K, L, M (A, B, C, D) | water | water | 18.0 | 77 | Chemie (HOH) |
Sequence modifications
A: 1 - 934 (UniProt: P43246)
B: 219 - 1134 (UniProt: P20585)
| PDB | External Database | Details |
|---|---|---|
| Ala 749 | Glu 749 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly 217 | - | expression tag |
| Pro 218 | - | expression tag |
| Arg 949 | Gln 949 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 223809.0 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 611.1 | |
| All* | Total formula weight | 224420.1 |
