8RB0
The crystal structure of DNA-bound human MutSbeta (MSH2_E749A/MSH3) in the canonical mismatch bound conformation with ADP bound in MSH2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-03-07 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 0.999965 |
| Spacegroup name | P 1 |
| Unit cell lengths | 66.426, 90.887, 96.293 |
| Unit cell angles | 67.59, 87.06, 74.56 |
Refinement procedure
| Resolution | 88.880 - 2.670 |
| R-factor | 0.24201 |
| Rwork | 0.241 |
| R-free | 0.31247 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.326 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.880 | 2.716 |
| High resolution limit [Å] | 2.670 | 2.670 |
| Number of reflections | 54597 | 2720 |
| <I/σ(I)> | 9.4 | |
| Completeness [%] | 96.0 | |
| Redundancy | 2.2 | |
| CC(1/2) | 0.997 | 0.843 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 0.1 M MES pH 6.5 -7.5, 0.2 M Ammonium Acetate, 20 - 25 % PEG 3350 |
