8R8R
Cryo-EM structure of the human mPSF with PAPOA C-terminus peptide (PAPOAc)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Cleavage and polyadenylation specificity factor subunit 1 | polymer | 1443 | 161074.2 | 1 | UniProt (Q10570) Pfam (PF10433) Pfam (PF03178) | Homo sapiens (human) | |
| 2 | B (B) | pre-mRNA 3' end processing protein WDR33 | polymer | 413 | 47580.1 | 1 | UniProt (Q9C0J8) Pfam (PF00400) | Homo sapiens (human) | |
| 3 | C (C) | Cleavage and polyadenylation specificity factor subunit 4 | polymer | 285 | 31733.4 | 1 | UniProt (O95639) Pfam (PF00642) Pfam (PF00098) Pfam (PF15663) | Homo sapiens (human) | |
| 4 | D (D) | cDNA FLJ50397, highly similar to Poly(A) polymerase alpha | polymer | 26 | 2872.4 | 1 | UniProt (B4DZL2) UniProt (by SIFTS) (P51003) | Homo sapiens | |
| 5 | E (E) | RNA (5'-R(P*AP*AP*UP*AP*AP*A)-3') | polymer | 6 | 1907.2 | 1 | Homo sapiens | ||
| 6 | F, G, H (C) | ZINC ION | non-polymer | 65.4 | 3 | Chemie (ZN) |
Sequence modifications
C: 2 - 244 (UniProt: O95639)
| PDB | External Database | Details |
|---|---|---|
| Met -40 | - | initiating methionine |
| Ser -39 | - | expression tag |
| Ala -38 | - | expression tag |
| Trp -37 | - | expression tag |
| Ser -36 | - | expression tag |
| His -35 | - | expression tag |
| Pro -34 | - | expression tag |
| Gln -33 | - | expression tag |
| Phe -32 | - | expression tag |
| Glu -31 | - | expression tag |
| Lys -30 | - | expression tag |
| Gly -29 | - | expression tag |
| Gly -28 | - | expression tag |
| Gly -27 | - | expression tag |
| Ser -26 | - | expression tag |
| Gly -25 | - | expression tag |
| Gly -24 | - | expression tag |
| Gly -23 | - | expression tag |
| Ser -22 | - | expression tag |
| Gly -21 | - | expression tag |
| Gly -20 | - | expression tag |
| Ser -19 | - | expression tag |
| Ala -18 | - | expression tag |
| Trp -17 | - | expression tag |
| Ser -16 | - | expression tag |
| His -15 | - | expression tag |
| Pro -14 | - | expression tag |
| Gln -13 | - | expression tag |
| Phe -12 | - | expression tag |
| Glu -11 | - | expression tag |
| Lys -10 | - | expression tag |
| Thr -9 | - | expression tag |
| Ala -8 | - | expression tag |
| Gly -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Glu -5 | - | expression tag |
| Val -4 | - | expression tag |
| Leu -3 | - | expression tag |
| Phe -2 | - | expression tag |
| Gln -1 | - | expression tag |
| Gly 0 | - | expression tag |
| Pro 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 245167.4 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 196.2 | |
| All* | Total formula weight | 245363.7 |
