8R70

Polysaccharide lyase BtPL33HA (BT4410) Y291A with HA dp4

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Heparinase	polymer	644	73568.5	2	UniProt (Q89ZG7) Pfam (PF07940)	Bacteroides thetaiotaomicron VPI-5482
2	C (C)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-beta-D-glucopyranuronic acid-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		600.5	1	In PDB GlyTouCan (G63629SO)
3	D (D)	beta-D-glucopyranuronic acid-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-beta-D-glucopyranuronic acid-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		776.6	1	In PDB GlyTouCan (G48124SB)
4	E, H (A, B)	polyethylene glycol	non-polymer		662.8	2	Chemie (P4K)
5	F (A)	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	1	Chemie (MES)
6	G, I (A, B)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
7	J, K (A, B)	water	water		18.0	700	Chemie (HOH)

Sequence modifications

A, B: 27 - 644 (UniProt: Q89ZG7)

PDB	External Database	Details
Met 1	-	initiating methionine
Gly 2	-	expression tag
Ser 3	-	expression tag
Ser 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
Ser 11	-	expression tag
Ser 12	-	expression tag
Gly 13	-	expression tag
Leu 14	-	expression tag
Val 15	-	expression tag
Pro 16	-	expression tag
Arg 17	-	expression tag
Gly 18	-	expression tag
Ser 19	-	expression tag
His 20	-	expression tag
Met 21	-	expression tag
Ala 22	-	expression tag
Ser 23	-	expression tag
Asn 24	-	expression tag
Ala 25	-	expression tag
Tyr 26	-	expression tag
Ala 291	Tyr 291	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	147136.9
Branched	Number of molecules	2
	Total formula weight	1377.2
Non-Polymers*	Number of molecules	5
	Total formula weight	1651.7
All*	Total formula weight	150165.7

*Water molecules are not included.