8R70
Polysaccharide lyase BtPL33HA (BT4410) Y291A with HA dp4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-07-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.95373 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.640, 137.301, 202.842 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 113.702 - 1.797 |
| Rwork | 0.181 |
| R-free | 0.22340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.215 |
| Data reduction software | xia2 |
| Data scaling software | STARANISO |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 113.702 | 1.893 |
| High resolution limit [Å] | 1.797 | 1.797 |
| Number of reflections | 122182 | 6109 |
| <I/σ(I)> | 14 | 1.4 |
| Completeness [%] | 95.4 | |
| Redundancy | 6.8 | |
| CC(1/2) | 0.999 | 0.698 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 20 % PEG 6000 0.1 M MES 0.1 M Ammonium chloride 20 mg/ml |
