8R65

1918 H1N1 Viral polymerase heterotrimer in complex with 4 repeat serine-5 phosphorylated PolII peptide with ordered PB2 C-terminal domains

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Polymerase acidic protein	polymer	716	82663.4	1	UniProt (Q3HM39)	Influenza A virus (A/Brevig Mission/1/1918(H1N1))
2	B (C)	Polymerase basic protein 2	polymer	905	102377.2	1	UniProt (Q3HM41)	Influenza A virus (A/Brevig Mission/1/1918(H1N1))
3	C (D)	RNA (5'-R(P*AP*GP*UP*AP*GP*AP*AP*AP*CP*AP*AP*GP*GP*CP*C)-3')	polymer	15	4862.0	1		Influenza A virus (A/Brevig Mission/1/1918(H1N1))
4	D (E)	RNA (5'-R(P*GP*GP*CP*CP*UP*GP*CP*U)-3')	polymer	17	5335.1	1		Influenza A virus (A/Brevig Mission/1/1918(H1N1))
5	E (X)	RNA polymerase II 4 repeat peptide with serine5 phosphorylation	polymer	28	3216.9	1	Pfam (PF05001) UniProt (by SIFTS) (P24928)	Homo sapiens
6	F (B)	RNA-directed RNA polymerase catalytic subunit	polymer	757	86625.2	1	UniProt (Q3HM40)	Influenza A virus (A/Brevig Mission/1/1918(H1N1))	Polymerase basic protein 1,PB1,RNA-directed RNA polymerase subunit P1

Sequence modifications

A: 1 - 716 (UniProt: Q3HM39)

PDB	External Database	Details
Ala 108	Asp 108	conflict

C: 1 - 759 (UniProt: Q3HM41)

PDB	External Database	Details
Glu 760	-	expression tag
Asn 761	-	expression tag
Leu 762	-	expression tag
Tyr 763	-	expression tag
Phe 764	-	expression tag
Gln 765	-	expression tag
Gly 766	-	expression tag
Glu 767	-	expression tag
Leu 768	-	expression tag
Lys 769	-	expression tag
Thr 770	-	expression tag
Ala 771	-	expression tag
Ala 772	-	expression tag
Leu 773	-	expression tag
Ala 774	-	expression tag
Gln 775	-	expression tag
His 776	-	expression tag
Asp 777	-	expression tag
Glu 778	-	expression tag
Ala 779	-	expression tag
Val 780	-	expression tag
Asp 781	-	expression tag
Asn 782	-	expression tag
Lys 783	-	expression tag
Phe 784	-	expression tag
Asn 785	-	expression tag
Lys 786	-	expression tag
Glu 787	-	expression tag
Gln 788	-	expression tag
Gln 789	-	expression tag
Asn 790	-	expression tag
Ala 791	-	expression tag
Phe 792	-	expression tag
Tyr 793	-	expression tag
Glu 794	-	expression tag
Ile 795	-	expression tag
Leu 796	-	expression tag
His 797	-	expression tag
Leu 798	-	expression tag
Pro 799	-	expression tag
Asn 800	-	expression tag
Leu 801	-	expression tag
Asn 802	-	expression tag
Glu 803	-	expression tag
Glu 804	-	expression tag
Gln 805	-	expression tag
Arg 806	-	expression tag
Asn 807	-	expression tag
Ala 808	-	expression tag
Phe 809	-	expression tag
Ile 810	-	expression tag
Gln 811	-	expression tag
Ser 812	-	expression tag
Leu 813	-	expression tag
Lys 814	-	expression tag
Asp 815	-	expression tag
Asp 816	-	expression tag
Pro 817	-	expression tag
Ser 818	-	expression tag
Gln 819	-	expression tag
Ser 820	-	expression tag
Ala 821	-	expression tag
Asn 822	-	expression tag
Leu 823	-	expression tag
Leu 824	-	expression tag
Ala 825	-	expression tag
Glu 826	-	expression tag
Ala 827	-	expression tag
Lys 828	-	expression tag
Lys 829	-	expression tag
Leu 830	-	expression tag
Asn 831	-	expression tag
Asp 832	-	expression tag
Ala 833	-	expression tag
Gln 834	-	expression tag
Ala 835	-	expression tag
Pro 836	-	expression tag
Lys 837	-	expression tag
Val 838	-	expression tag
Asp 839	-	expression tag
Asn 840	-	expression tag
Lys 841	-	expression tag
Phe 842	-	expression tag
Asn 843	-	expression tag
Lys 844	-	expression tag
Glu 845	-	expression tag
Gln 846	-	expression tag
Gln 847	-	expression tag
Asn 848	-	expression tag
Ala 849	-	expression tag
Phe 850	-	expression tag
Tyr 851	-	expression tag
Glu 852	-	expression tag
Ile 853	-	expression tag
Leu 854	-	expression tag
His 855	-	expression tag
Leu 856	-	expression tag
Pro 857	-	expression tag
Asn 858	-	expression tag
Leu 859	-	expression tag
Asn 860	-	expression tag
Glu 861	-	expression tag
Glu 862	-	expression tag
Gln 863	-	expression tag
Arg 864	-	expression tag
Asn 865	-	expression tag
Ala 866	-	expression tag
Phe 867	-	expression tag
Ile 868	-	expression tag
Gln 869	-	expression tag
Ser 870	-	expression tag
Leu 871	-	expression tag
Lys 872	-	expression tag
Ala 873	-	expression tag
Asp 874	-	expression tag
Pro 875	-	expression tag
Ser 876	-	expression tag
Gln 877	-	expression tag
Ser 878	-	expression tag
Ala 879	-	expression tag
Asn 880	-	expression tag
Leu 881	-	expression tag
Leu 882	-	expression tag
Ala 883	-	expression tag
Glu 884	-	expression tag
Ala 885	-	expression tag
Lys 886	-	expression tag
Lys 887	-	expression tag
Leu 888	-	expression tag
Asn 889	-	expression tag
Gly 890	-	expression tag
Ala 891	-	expression tag
Gln 892	-	expression tag
Ala 893	-	expression tag
Pro 894	-	expression tag
Lys 895	-	expression tag
Val 896	-	expression tag
Asp 897	-	expression tag
Ala 898	-	expression tag
Asn 899	-	expression tag
Ser 900	-	expression tag
Ala 901	-	expression tag
Gly 902	-	expression tag
Lys 903	-	expression tag
Ser 904	-	expression tag
Thr 905	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	285079.8
All*	Total formula weight	285079.8

*Water molecules are not included.