8R64

Cryo-EM structure of the FIGNL1 AAA hexamer bound to RAD51

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (B, C, D, E, F...)	Fidgetin-like protein 1	polymer	417	46154.5	6	UniProt (Q6PIW4) Pfam (PF00004) Pfam (PF17862) Pfam (PF09336)	Homo sapiens (human)
2	G (G)	DNA repair protein RAD51 homolog 1	polymer	339	37009.1	1	UniProt (Q06609) Pfam (PF14520) Pfam (PF08423)	Homo sapiens (human)	HsRAD51,hRAD51,RAD51 homolog A
3	H, J, L, N, P... (B, C, D, E, F...)	MAGNESIUM ION	non-polymer		24.3	6	Chemie (MG)
4	I, K, M, O, S (B, C, D, E, A)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	5	Chemie (ATP)
5	Q (F)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)

Sequence modifications

B, C, D, E, F, A: 284 - 674 (UniProt: Q6PIW4)

PDB	External Database	Details
Met 268	-	initiating methionine
Ala 269	-	expression tag
His 270	-	expression tag
His 271	-	expression tag
His 272	-	expression tag
His 273	-	expression tag
His 274	-	expression tag
His 275	-	expression tag
Ser 276	-	expression tag
Ser 277	-	expression tag
Gly 278	-	expression tag
Leu 279	-	expression tag
Glu 280	-	expression tag
Val 281	-	expression tag
Leu 282	-	expression tag
Phe 283	-	expression tag
Gln 284	Asn 284	conflict
Gln 501	Glu 501	engineered mutation
Ser 675	-	expression tag
Ala 676	-	expression tag
Trp 677	-	expression tag
Ser 678	-	expression tag
His 679	-	expression tag
Pro 680	-	expression tag
Gln 681	-	expression tag
Phe 682	-	expression tag
Glu 683	-	expression tag
Lys 684	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	313936.1
Non-Polymers*	Number of molecules	12
	Total formula weight	3108.9
All*	Total formula weight	317045.1

*Water molecules are not included.