8R0S

Structure of reverse transcriptase from Cauliflower Mosaic Virus in complex with RNA/DNA hybrid

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Enzymatic polyprotein	polymer	577	67140.3	1	UniProt (A0A2D2PYL0)	Cauliflower mosaic virus
2	B (C)	RNA (5'-R(*GP*UP*CP*CP*AP*GP*CP*AP*GP*UP*GP*CP*GP*UP*AP*GP*C)-3')	polymer	17	5458.3	1		synthetic gene
3	C (D)	DNA (5'-D(*GP*CP*TP*AP*CP*GP*CP*AP*CP*TP*GP*CP*TP*GP*GP*A)-3')	polymer	16	4899.2	1		synthetic gene
4	D, E, F (A, C, D)	water	water		18.0	74	Chemie (HOH)

Sequence modifications

A: 1 - 475 (UniProt: A0A2D2PYL0)

PDB	External Database	Details
Met -101	-	initiating methionine
Gly -100	-	expression tag
Ser -99	-	expression tag
Ser -98	-	expression tag
His -97	-	expression tag
His -96	-	expression tag
His -95	-	expression tag
His -94	-	expression tag
His -93	-	expression tag
His -92	-	expression tag
Ser -91	-	expression tag
Ser -90	-	expression tag
Gly -89	-	expression tag
Leu -88	-	expression tag
Val -87	-	expression tag
Pro -86	-	expression tag
Arg -85	-	expression tag
Gly -84	-	expression tag
Ser -83	-	expression tag
His -82	-	expression tag
Met -81	-	expression tag
Glu -80	-	expression tag
Gly -79	-	expression tag
Glu -78	-	expression tag
Tyr -77	-	expression tag
Ile -76	-	expression tag
Lys -75	-	expression tag
Leu -74	-	expression tag
Lys -73	-	expression tag
Val -72	-	expression tag
Ile -71	-	expression tag
Gly -70	-	expression tag
Gln -69	-	expression tag
Asp -68	-	expression tag
Ser -67	-	expression tag
Ser -66	-	expression tag
Glu -65	-	expression tag
Ile -64	-	expression tag
His -63	-	expression tag
Phe -62	-	expression tag
Lys -61	-	expression tag
Val -60	-	expression tag
Lys -59	-	expression tag
Met -58	-	expression tag
Thr -57	-	expression tag
Thr -56	-	expression tag
His -55	-	expression tag
Leu -54	-	expression tag
Lys -53	-	expression tag
Lys -52	-	expression tag
Leu -51	-	expression tag
Lys -50	-	expression tag
Glu -49	-	expression tag
Ser -48	-	expression tag
Tyr -47	-	expression tag
Cys -46	-	expression tag
Gln -45	-	expression tag
Arg -44	-	expression tag
Gln -43	-	expression tag
Gly -42	-	expression tag
Val -41	-	expression tag
Pro -40	-	expression tag
Met -39	-	expression tag
Asn -38	-	expression tag
Ser -37	-	expression tag
Leu -36	-	expression tag
Arg -35	-	expression tag
Phe -34	-	expression tag
Leu -33	-	expression tag
Phe -32	-	expression tag
Glu -31	-	expression tag
Gly -30	-	expression tag
Gln -29	-	expression tag
Arg -28	-	expression tag
Ile -27	-	expression tag
Ala -26	-	expression tag
Asp -25	-	expression tag
Asn -24	-	expression tag
His -23	-	expression tag
Thr -22	-	expression tag
Pro -21	-	expression tag
Lys -20	-	expression tag
Glu -19	-	expression tag
Leu -18	-	expression tag
Gly -17	-	expression tag
Met -16	-	expression tag
Glu -15	-	expression tag
Glu -14	-	expression tag
Glu -13	-	expression tag
Asp -12	-	expression tag
Val -11	-	expression tag
Ile -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Tyr -7	-	expression tag
Gln -6	-	expression tag
Glu -5	-	expression tag
Gln -4	-	expression tag
Thr -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Asn 28	Ser 224	conflict
Asn 421	Asp 617	conflict

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	77497.8
All*	Total formula weight	77497.8

*Water molecules are not included.